Study on Key Quality Control Indicators and Antioxidant Activity of Typical Propolis Samples from domestic and Abroad
10.14148/j.issn.1672-0482.2025.0522
- VernacularTitle:国内外典型蜂胶样本关键质控指标及抗氧化活性研究
- Author:
Mengyuan WANG
1
;
Dongping YUAN
;
Qiumei WU
;
Xiaowen RUAN
;
Yuchen JIANG
;
Jiwen YANG
;
Tao LI
;
Xuan LI
Author Information
1. 南京中医药大学药学院,江苏 南京 210023
- Publication Type:Journal Article
- Keywords:
propolis;
ethanol extract;
total phenols;
total flavonoids;
antioxidant
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2025;41(4):522-530
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To analyze the ethanol extracts,total phenols,total flavonoids contents and HPLC fingerprints of typi-cal propolis samples from 6 foreign countries and 5 domestic regions,optimize the extraction process and evaluate the antioxidant activi-ty,so as to provide data support for improving the quality control system of propolis.METHODS The optimization of the propolis ex-traction process utilized flavonoid content as an indicator.Three flavonoid detection methods-namely,the aluminum trichloride meth-od,aluminum nitrate method,and polyamide method-were compared.The content of ethanol extract,total phenol content,and the scavenging ability of DPPH free radicals for each sample were determined.Further analysis was conducted using HPLC fingerprint pro-filing.RESULTS The propolis extract with the highest flavonoid content was obtained using 80%ethanol as the extraction solvent,operating at 50℃,with a stirring time of 3 h,ultrasonic power of 180 W,and ultrasonic time of 15 min.The aluminum trichloride method was proved to be the most effective for determining total flavonoids in propolis.While the ethanol extract,total flavonoids,and total phenols of propolis from Xinjiang,China were relatively low,their antioxidant activity exhibited superiority.HPLC analysis re-vealed,Brazilian red propolis lacked of chrysin,galangin,caffeic acid phenethyl ester and Brazilian green propolis lacked ferulic acid,apigenin,p-coumaric acid,chrysin,and pinocembrin.In contrast,the content of these four compounds in other samples varied,with the antioxidant capacity of the extracts not precisely corresponding to the compound content.CONCLUSION Propolis exhibits a complex chemical composition with significant variations among varieties.Key quality control indexes must be comprehensively consid-ered,encompassing physicochemical characteristics and biological activity.Establishing a multi-angle assessment system with a mate-rial basis-functional linkage is essential.This approach facilitates the realization of high quality and cost-effectiveness,thereby promo-ting the healthy development of the industry.
