Effects of exosomal miR-151A-3P from colorectal cancer on proliferation,migration and prognosis of colorectal cancer cells
10.11904/j.issn.1002-3070.2025.02.008
- VernacularTitle:结直肠癌来源外泌体miR-151A-3P对结直肠癌细胞增殖、迁移及患者预后的影响
- Author:
He LIU
1
;
Yang PAN
1
;
Xiaolong LIANG
1
;
Zhengqiang WEI
1
Author Information
1. 重庆医科大学附属第一医院胃肠外科(重庆 400016)
- Publication Type:Journal Article
- Keywords:
Colorectal cancer;
Exosome;
miR-151A-3P;
Proliferation;
Migration
- From:
Practical Oncology Journal
2025;39(2):126-133
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to investigate the key genes that regulate colorectal cancer(CRC)metasta-sis,evaluate the effects of exosomal miR-151A-3P derived from CRC on the proliferation and migration,and explore the possibility of exosomal miR-151A-3P as a prognostic biomarker for CRC patients.Methods High-throughput sequencing was performed to screen the differentially expressed miRNAs in serum exosomes of metastatic CRC patients.RT-qPCR was used to verify the expression of differentially expressed miRNAs in CRC specimens.After mimics overexpression or knockdown of miR-151A-3P,CCK-8 and EdU assays were used to detect cell proliferation,and Scratch wound healing and Transwell assays were used to detect cell migration and in-vasion capability.A BALB/c nude mouse model was used to evaluate the effect of exosomal miR-151A-3P on the metastatic ability of CRC cells.Results The sequencing results showed that compared with non-metastatic CRC patients,the expression of miR-151A-3P was significantly increased in serum exosomes of metastatic CRC patients(P<0.001).The results were validated by RT-qPCR and found that miR-151A-3P was highly expressed in both serum exosomes and tumor tissues of metastatic CRC patients(P<0.001).The overall survival of CRC patients with high expression of serum exosomal miR-151A-3P was shorter than that of CRC pa-tients with low expression(P=0.029).After silencing miR-151A-3P,the cell proliferation,migration,and invasion abilities of CRC cells were decreased(P<0.05),while overexpression of miR-151A-3P could promote the proliferation,migration and invasion of CRC cells(P<0.05).In the BALB/c nude mouse colorectal cancer model,the administration of exosomal miR-151A-3P led to an increase in the number of lung metastases in BALB/c nude mice(P<0.05).Conclusion CRC-derived exosomal miR-151A-3P promotes the proliferation and migration of CRC cells,and is associated with poor prognosis of CRC patients.
