Effect and molecular mechanism of RAB11B antisense RNA1 on gemcitabine-resistant colorectal cancer cells
10.3760/cma.j.cn115807-20231116-00155
- VernacularTitle:RAB11B-AS1对耐吉西他滨结直肠癌细胞的影响及分子机制
- Author:
Yuqing YUAN
1
;
Yucheng ZHANG
;
Yexiao HU
;
Yanyan ZHENG
Author Information
1. 温州市中西医结合医院肛肠科，温州 325000
- Publication Type:Journal Article
- Keywords:
LncRNA RAB11B-AS1;
miR-486-5p;
Gemcitabine;
Colorectal cancer;
Drug resistance
- From:
Chinese Journal of Endocrine Surgery
2024;18(6):875-880
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect and molecular mechanism of long non-coding RNA (LncRNA) RAB11B-AS1 on the proliferation and apoptosis of colorectal cancer cells.Methods:Cancerous tissues and para-cancerous tissues of 37 patients with colorectal cancer from Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine from Jan. 2017 to Jan. 2019 were selected. By gradually increasing the concentration of gemcitabine in the medium, the colorectal cancer cell Caco-2-Gem resistant to gemcitabine was established and divided into si-NC group, si-RAB11B-AS1 group, si-RAB11B-AS1+anti-miR-NC group, and si-RAB11B-AS1+ anti-miR-486-5p group. The expression of miR-486-5p and RAB11B-AS1 was detected by real-time quantitative PCR (RT-qPCR) ; Caco-2 and Caco-2-Gem cells were treated with 0, 1, 5, 10, 50, 100, and 500 μmol/L gemcitabine, respectively; Cell viability was measured by MTT assay; The cell cycle and apoptosis were detected by flow cytometry.Results:Compared with Caco-2 cells and adjacent tissues, the expressions of RAB11B-AS1 was increased in colorectal cancer tissues and Caco-2-Gem cells ( P<0.05), while the expression of miR-486-5p was decreased ( P<0.05). Compared with the 0 μmol/L group, the survival rate of Caco-2 cells after 1-100 μmol/L gemcitabine treatment was significantly reduced ( P<0.05) ; the survival rate of Caco-2-Gem cells had no significant change after 1-10 μmol/L gemcitabine treatment, and the survival rate of Caco-2-Gem cells was decreased after treatment with Gissi (50-500μmol/L) ( P<0.05). After inhibiting the expression of LncRNA RAB11B-AS1, the survival rate of Caco-2-Gem cells decreased, the apoptosis rate increased, the proportion of cells in S phase decreased, and the proportion of cells in G0-G1 phase increased. After inhibiting the expression of LncRNA RAB11B-AS1 and down-regulating the expression of miR-486-5p, the survival rate of Caco-2-Gem cells was increased, the apoptosis rate was decreased, the proportion of cells in S phase was increased, and the proportion of cells in G0-G1 phase was decreased ( P<0.05) . Conclusions:Inhibition of the expression of the lncRNA RAB11B-AS1 may have a negative regulatory effect on miR-486-5p, thereby inhibiting proliferation while promoting apoptosis in colorectal cancer cells resistant to Gissi, thus improving the biological efficacy of Gissi on colorectal cancer cells.
