Effect of Astragalus polysaccharides on intestinal immune inflammation of rats with dampness stagnancy due to spleen deficiency syndrome based on fecal microbiota transplantation
10.3969/j.issn.1000-484X.2025.05.019
- VernacularTitle:基于粪菌移植探讨黄芪多糖对脾虚水湿不化模型大鼠肠道免疫炎症的影响
- Author:
Jun ZHENG
1
;
Chenchen DUAN
;
Qin LYU
;
Xuelian ZHAO
;
Binbin YANG
;
Shijun WANG
;
Wenxiao ZHAO
Author Information
1. 山东中医药大学护理学院,济南 250355
- Publication Type:Journal Article
- Keywords:
Fecal microbiota transplantation;
Astragalus polysaccharides;
TLR4/NF-κB pathway;
Dampness stagnancy due to spleen deficiency syndrome;
Intestinal flora;
Immune inflammation
- From:
Chinese Journal of Immunology
2025;41(5):1135-1139
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore effect of Astragalus polysaccharides(APS)through TLR4/NF-κB signal pathway on intesti-nal immune inflammation of rats with dampness stagnancy due to spleen deficiency syndrome based on fecal microbiota transplantation.Methods:Rats with dampness stagnancy due to spleen deficiency syndrome were fed with high-fat and low-protein diet and subjected to exhaustive swimming,transplant flora microbiota after intervention of APS.General condition,changes in weight gain,time of weight exhaustion swimming,spleen index and thymus index were tested.Pathological manifestations of duodenal tissue inflammation were observed by HE staining.Serum IL-1β,IL-6 and TNF-α concentrations of rats were determined by ELISA.Protein levels of TLR4,NF-κB p65 and IκBα in colon were determined by Western blot.Results:Compared with model group,general condition rating,changes in weight gain and time of weight exhaustion swimming in APS-FTG group were increased significantly(P<0.01);spleen index and thymus index were increased;pathological manifestations of duodenal inflammation were significantly relieved;serum IL-1β,IL-6 and TNF-α concentrations were decreased significantly(P<0.01),TLR4 and NF-κB p65 levels in colon were decreased significantly(P<0.05 and P<0.01),IκBα level was in colon increased significantly(P<0.01).Conclusion:APS improves intestinal microbiota of rats with dampness stagnancy due to spleen deficiency syndrome and inhibits TLR4/NF-κB pathway activation through intestinal flora,thus improving intestinal immune inflammation status in rats with dampness stagnancy due to spleen deficiency syndrome.
