Analysis of endometrial microbiota characteristics in patients with varying degrees of intrauterine adhesions
10.3760/cma.j.cn101441-20250715-00296
- VernacularTitle:不同程度宫腔粘连患者子宫内膜菌群特征分析
- Author:
Yiyang LUO
1
;
Zhoulin ZHANG
;
Yu XIAO
;
Qiaoyun ZHOU
;
Wenjun JIANG
;
Wanfeng SONG
;
Tianyu MIAO
;
Xin AN
;
Xiaowu HUANG
Author Information
1. 首都医科大学附属复兴医院宫腔镜诊治中心，北京 100038
- Publication Type:Journal Article
- Keywords:
Lactobacillus;
16S rRNA sequencing;
Intrauterine adhesion;
Endometrial microbiota;
Dysbiosis
- From:
Chinese Journal of Reproduction and Contraception
2025;45(9):880-885
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the characteristics of the endometrial microbiota in patients with varying degrees of intrauterine adhesion (IUA).Methods:This single-center cross-sectional observational study enrolled 115 patients with IUA who were treated at the Hysteroscopic Center of Fuxing Hospital, Capital Medical University, from May 2022 to October 2023. After quality control and data preprocessing, 81 samples met the inclusion criteria for analysis. Patients were grouped according to an established IUA scoring and grading system into mild IUA ( n=38) and moderate-to-severe IUA ( n=43). Endometrial tissue was collected under sterile conditions. Bacterial genomic DNA was extracted, the 16S rRNA V3-V4 region was amplified, and sequencing was performed on an Illumina platform. Differences in endometrial microbiota diversity and composition were compared between the two groups. Results:Patients with varying degrees of IUA exhibited comparable species richness, evenness and diversity of endometrial microbiota. At the phylum level, the endometrial microbiota across all subjects was predominantly composed of Proteobacteria, Firmicutes, Cyanobacteriota, Bacteroidota, and Actinobacteriota, with Proteobacteria (32.29%) and Firmicutes (23.82%) showing the highest mean relative abundances. At the genus level, Ralstonia (16.67%), Lactobacillus (13.45%), and Streptococcus (7.07%) were the most abundant genera. Group comparisons showed that the abundance of Ralstonia was higher in the mild IUA group, whereas Lactobacillus, Vibrio and Pseudoalteromonas were more abundant in the moderate-to-severe IUA group; however, these differences did not reach statistical significance (all P>0.05). LEfSe analysis further indicated that Lactobacillus, Vibrio, Pseudoalteromonas, Aeromonas, Ureaplasma and Acetobacterium were relatively enriched in the moderate-to-severe IUA group, while Geobacillus, Stomatobaculum and Fusicatenibacter were more abundant in the mild IUA group. Conclusion:The composition of the endometrial microbiota differs among patients with varying IUA severity. IUA progression may be associated with alterations in the endometrial microbiota; however, causal relationships and underlying mechanisms require further investigation.
