Immunophenotypic analysis of spleen in PD-L2 gene knockout mice
10.3969/j.issn.1000-484X.2025.05.005
- VernacularTitle:PD-L2基因敲除小鼠的脾脏免疫表型分析
- Author:
Yanhong YANG
1
;
Saizhe SONG
;
Cuiping LIU
Author Information
1. 苏州大学附属第一医院江苏省临床免疫研究所,苏州 215021;苏州大学附属第二医院妇产科,苏州 215004
- Publication Type:Journal Article
- Keywords:
PD-L2;
Gene knockout;
Tissue structure;
Immune phenotype;
Inflammatory response
- From:
Chinese Journal of Immunology
2025;41(5):1048-1054
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate impact of PD-L2 gene knockout on splenic immune phenotype of C57BL/6 mice.Methods:Body weight of female and male wild-type(WT)and PD-L2 gene knockout(KO)mice aged 3～12 weeks were continuously measured.Tissue structure differences in heart,liver,spleen,lung,kidney,thymus,ovary,uterus and testis of four groups of mice were observed by HE staining.Urease UV rate method was employed to measure urea level in mouse serum,and creatinine concentra-tion in serum was determined by creatine oxidase method.Flow cytometry was used to assess proportions of immune cells and cytokine differences in spleens of mice.Results:PD-L2 gene knockout had no apparent effect on body weight or organ structure of mice.Serum urea and creatinine levels were higher in female KO mice compared to WT mice,but no significant differences were observed among males.Female KO mice exhibited reduced proportions of CD4+T cells,CD8+T cells,Treg,NK cells and dendritic cells(DC)in spleen compared to WT mice.Conversely,proportions of Tfh,B cells,plasma cells,na?ve B cells and B2 cells were elevated,proin-flammatory cytokines such as IL-17,IL-6 and IL-22 were increased,while anti-inflammatory cytokines like IL-10 and IL-4 were decreased.Differences among male mice were either not significant or absent.Conclusion:PD-L2 gene knockout primarily affects splenic immune phenotype of female mice,leading to increased inflammatory responses and weakened immune suppression after knockout.
