Effect of miR-22-3p regulation of NF-κB signaling pathway on palmitic acid-induced inflammation injury and apoptosis of hepatocytes
10.3969/j.issn.1000-484X.2025.05.003
- VernacularTitle:miR-22-3p调控NF-κB信号通路对棕榈酸诱导的肝细胞炎症损伤和凋亡的影响
- Author:
Yunchun XU
1
;
Xinya YU
1
;
Yuwei LI
1
;
Le GUO
1
Author Information
1. 大理大学基础医学院,大理 671000
- Publication Type:Journal Article
- Keywords:
miR-22-3p;
Lipid toxicity;
Hepatocyte injury;
Inflammation;
Apoptosis;
NF-κB signaling pathway
- From:
Chinese Journal of Immunology
2025;41(5):1035-1040
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role and potential regulation mechanism of miR-22-3p in lipotoxic hepatocyte inflam-matory injury and apoptosis caused by palmitic acid(PA).Methods:Human normal immortalized hepatocytes(LO2 cells)were treated with different concentrations of PA for 24 h.CCK-8 and qRT-PCR were used to detect cell proliferation and miR-22-3p expression.miR-22-3p mimics or inhibitors were transfected into LO2 cells and then treated with 0.32 mmol/L PA for 24 h,the expression level of miR-22-3p was determined by qRT-PCR;cell viability was determined by CCK-8;biochemical kits to determine the ALT and AST contents;the mRNA and protein expression levels of inflammatory cytokines TNF-α,IL-1β and IL-6 in intracellular and culture super-natants were determined by qRT-PCR and ELISA;the cell apoptosis rate in each group was determined by flow cytometry;the expres-sion levels of NF-κB signaling pathway-related proteins were detected by Western blot and immunofluorescence.Results:With the increaseing of PA concentration,the cell survival rate and the expression of miR-22-3p decreased in a dose-dependent manner.After PA treatment,the cell proliferation activity decreased significantly,the activities of ALT and AST enzymes were increased,the expressions of inflammatory cytokines TNF-α,IL-1β and IL-6 were increased,cell apoptosis was increased,and NF-κB signaling pathway was activated.Transfection of miR-22-3p mimics significantly increased the proliferation activity of LO2 cells,and decreased the levels of ATL,AST,TNF-α,IL-1β,IL-6 and apoptosis,and inhibited the activation of NF-κB signaling pathway.Transfection of miR-22-3p inhibitor further activated NF-κB signaling pathway,and promoted cell inflammatory injury and apoptosis(all P<0.05).Conclusion:Overexpression of miR-22-3p can alleviate PA-induced apoptosis and inflammation,and the mechanism is related to inhibit the activation of NF-κB signaling pathway.
