Effects and mechanisms of CD147 on differentiation of circulating fibrocytes and its pro-inflammatory and pro-fibrotic function
10.3969/j.issn.1000-484X.2025.05.008
- VernacularTitle:CD147对循环纤维细胞分化及促炎促纤维化功能的影响及机制研究
- Author:
Jincai ZHOU
1
;
Zhao LI
1
;
Tao CHENG
1
;
Zexiang LIN
1
;
Jing WANG
1
;
Xinpeng HAN
1
Author Information
1. 西安国际医学中心医院呼吸与危重症医学科,西安 710100
- Publication Type:Journal Article
- Keywords:
CD147;
Airway remodeling;
Circulating fibrocytes;
p38/MAPK
- From:
Chinese Journal of Immunology
2025;41(5):1066-1071
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect and mechanism of CD147 on differentiation of circulating fibrocytes and its pro-inflammatory and pro-fibrotic function.Methods:Circulating fibrocytes were extracted from peripheral blood of asthma patients,stimu-lated with CD147,and intervened with CD147 antagonist AP-9 or p38/MAPK inhibitor SB203580.Cell viability was detected by CCK-8 method,cell migration was assessed by Transwell method,expressions of collagen type Ⅰ(COL-Ⅰ),CD45 and α-SMA were detected by immunofluorescence staining.Levels of cytokines such as TGF-β1,IL-4,IL-5 and IL-13 were detected by ELISA,and expression levels of COL-Ⅰ,fibronectin(FN),α-SMA,p-TAK1,p-P38 and other proteins were detected by Western blot.Results:Low dose of CD147 increased cell viability of circulating fibrocytes.CD147 significantly promoted migration of circulating fibrocytes and induces their differentiation into myofibrocytes.CD147 significantly increased secretion of cytokines such as TGF-β1,IL-4,IL-5 and IL-13 in circulating fibrocytes.CD147 significantly increased expressions of COL-Ⅰ,FN,α-SMA,p-TAK1,p-P38 in circulating fibrocytes.AP-9 or SB203580 significantly inhibited effect of CD147 on circulating fibrocytes.Conclusion:CD147 can promote proliferation,mi-gration,differentiation and pro-inflammatory and profibrotic function of circulating fibrocytes in vitro,and TAK1-p38/MAPK signaling pathway is involved in CD147-mediated regulation of circulating fibrocytes.
