Impact of Lupeol on Th17/Treg immune balance in rats with ulcerative colitis by regulating PI3K/AKT/NF-κB signal pathway
10.3969/j.issn.1000-484X.2025.05.007
- VernacularTitle:羽扇豆醇调节PI3K/AKT/NF-κB信号通路对溃疡性结肠炎大鼠Th17/Treg免疫平衡的影响
- Author:
Huidong LU
1
;
Yanmei LI
Author Information
1. 赤峰市医院消化内科,赤峰 024000
- Publication Type:Journal Article
- Keywords:
Lupeol;
PI3K/AKT/NF-κB signal pathway;
Ulcerative colitis;
Th17/Treg;
Immune balance
- From:
Chinese Journal of Immunology
2025;41(5):1060-1065
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the impact of Lupeol on Th17/Treg immune balance in rats with ulcerative colitis(UC)and the regulatory mechanism on PI3K/AKT/NF-κB signal pathway.Methods:All rats were randomly grouped into control group,model group,positive drug group(sulfasalazine),PI3K inhibitor group(LY294002 group),Lupeol group and Lupeol+PI3K activator group(Lupeol+740Y-P group),with 15 rats in each group.The rats were scored with UC disease activity index(DAI);HE staining was used to observe the pathological changes of the colon in rats and inflammation scoring was performed;the levels of serum IL-17 and IL-10 were detected by ELISA;the levels of Th17 and Treg cells in the mucosa of lamina propria of colon were detected by flow cytome-try;Western blot was applied to detect the expressions of RORγt,FOXP3 and PI3K/AKT/NF-κB pathway related proteins in colon tis-sue.Results:Compared with the control group,DAI score,inflammation score,serum IL-17 content,colon tissue Th17 cell level,RORγt expression and p-PI3K/PI3K,p-AKT/AKT,p-NF-κB p65/NF-κB p65 of rats in model group were increased,IL-10 content,Treg cell level and FOXP3 expression were decreased(P<0.05);compared with the model group,DAI score,inflammation score,serum IL-17 content,colon tissue Th17 cell level,RORγt expression and p-PI3K/PI3K,p-AKT/AKT,p-NF-κB p65/NF-κB p65 of rats in positive drug group,LY294002 group and Lupeol group were decreased,IL-10 content,Treg cell level and FOXP3 expression were increased(P<0.05);after further addition of PI3K activator 740Y-P,it was found that 740Y-P reversed the inhibition of Lupeol on Th17 cells and the promotion on Treg cells(P<0.05).Conclusion:Lupeol regulates Th17/Treg immune balance in UC rats by inhibiting PI3K/AKT/NF-κB signal pathway.
