Exploring reference interval of IL-10 in cerebrospinal fluid of adults by multiplex bead-based flow fluorescent immunoassay
10.3969/j.issn.1000-484X.2025.05.026
- VernacularTitle:多重微球流式荧光免疫技术检测成人脑脊液IL-10水平参考区间探索
- Author:
Dongmei ZOU
1
;
Yan LIU
;
Jiangang DUAN
;
Zheng LIU
;
Yixian GUO
;
Wanling SUN
Author Information
1. 首都医科大学宣武医院血液科,北京 100053
- Publication Type:Journal Article
- Keywords:
Multiplex bead-based flow fluorescent immunoassay;
Cerebrospinal fluid;
IL-10;
Reference interval
- From:
Chinese Journal of Immunology
2025;41(5):1182-1185,1191
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the reference interval of IL-10 level in cerebrospinal fluid of adults using multiplex bead-based flow fluorescent immunoassay(MBFFI).Methods:A total of 743 patients without tumor were involved and grouped by diagno-sis.Cerebrospinal fluid and plasma IL-10 were tested by MBFFI.Results:①Cerebrospinal fluid IL-10 levels of the central nervous system infection group were higher than those of other groups.②The cranial venous sinus thrombosis(CVST)group without tumor,in-fection or inflammatory disease were chosen as nearly normal population.As the distribution of cerebrospinal fluid IL-10 levels in these 250 CVST patients were skewed,95％cut-off level was chosen as upper limit and cerebrospinal fluid IL-10<3.50 pg/ml was the refer-ence interval.③No correlation was found between plasma and cerebrospinal fluid IL-10 levels in the CVST group.Conclusion:The medical reference interval of cerebrospinal fluid IL-10 by MBFFI in adults are determined in this study,that is<3.50 pg/ml,to pro-vide clinical reference for practical applications.
