Application of artificial rearing technology in cultivating gnotobiotic rodents
10.3969/j.issn.1005-4847.2025.03.014
- VernacularTitle:人工哺乳技术在啮齿无菌动物培育中的应用
- Author:
Xiaopeng DU
1
;
Yaxi GUO
1
;
Hua ZHU
1
Author Information
1. 中国医学科学院医学实验动物研究所,国家动物模型技术创新中心,国家卫生健康委比较医学重点实验室,国家人类疾病动物模型资源库,北京 100021
- Publication Type:Journal Article
- Keywords:
germ-free animal;
artificial rearing;
milk substitute
- From:
Acta Laboratorium Animalis Scientia Sinica
2025;33(3):457-466
- CountryChina
- Language:Chinese
-
Abstract:
In recent years,germ-free rats and mice have received extensive attention and application in the field of biomedical research,particularly in gut microbiota studies.The demand for germ-free rodents has been increasing,requiring many research institutions to establish quality germ-free animal populations.The process of breeding first-generation germ-free animals involves highly specialized and technically challenging work,such as using artificial rearing techniques to feed rodent pups.This review article provides an overview of key aspects of artificial rearing techniques,including nursing method,preparation of artificial milk,and sterilization method.It retrospectively summarizes the key technical points and,based on this foundation,offers prospects for further applications of artificial rearing techniques.
