Construction of a mouse model to trace neurons and microglia and ablate microglia for investigating their interactive roles
- VernacularTitle:神经元与小胶质细胞示踪及可清除小鼠模型构建
- Author:
Mei CHEN
1
;
Zhun-hong ZHANG
;
Shi-feng CHU
;
Zhao ZHANG
;
Nai-hong CHEN
Author Information
- Publication Type:Journal Article
- Keywords: microglia; neurons; brain homeostasis; in vivo visu-alization; two-photon imaging; central nervous system diseases
- From: Chinese Pharmacological Bulletin 2025;41(5):994-999
- CountryChina
- Language:Chinese
- Abstract: Aim To generate a dual-reporter mouse model for tracing microglia and neurons to analyze their dynamic changes in real-time and to investigate the functional role of microglia-neuron interactions through microglial depletion.Methods Mi-croglia reporter mice were crossed with neuron reporter mice to generate Tmem119-e(2A-tdTomato-2A-DTR);Thy1-GFP dual-reporter mice.Genotyping was performed using tail DNA.Con-focal and two-photon microscopy were used to examine neuronal morphology.In vivo two-photon imaging was employed to observe the distribution of microglia and neurons in the brain.Diphtheria toxin(DT)was intraperitoneally injected to deplete microglia.Results The dual-reporter mice were successfully generated with the correct genotype.Both microglia and neurons were visu-alized,and microglia were specifically depleted by DT.Conclu-sions The successful establishment of a dual-reporter mouse model for tracing neurons and microglia will facilitate real-time in vivo observation of microglial and neuronal changes under physiological or pathological conditions,elucidating their specific roles in brain homeostasis.
