Hyperoside regulates energy metabolism and apoptosis in cardiomyocytes after myocardial infarctionby activation of PPARα signaling pathway
- VernacularTitle:金丝桃苷通过激活PPARα信号通路调控心肌梗死后心肌细胞能量代谢和凋亡
- Author:
Jia-hao HUANG
1
;
Hua TONG
1
;
Yan-yan YIN
1
;
Jun-yan ZHANG
1
Author Information
- Publication Type:Journal Article
- Keywords: hyperoside; cardiac remodeling; myocardi-al infarction; energy metabolism; apoptosis; PPARα signaling pathway
- From: Chinese Pharmacological Bulletin 2025;41(5):908-916
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the relation between the protective effect of hyperoside(Hyp)on energy metab-olism and apoptosis in cardiomyocytes after myocardial infarction(MI)and the regulation of peroxisome pro-life rator-activated receptor α(PPARα)pathway.Methods Mouse myocardial infarction injury model was established by ligation of left anterior descending coronary artery.The mice that were successfully liga-ted were randomly divided into the following groups:MI group,Hyp(9,18 and 36 mg·kg-1)group,posi-tive fenofibrate group(120 mg·kg-1)and Hyp(36 mg·kg-1)+PPARα inhibitor GW6471 group.In addition,a sham group was set up,only threading with-out ligature.The mice were administered different kinds of drugs by gavage for seven days,once daily.ECG changes were recorded in mice 5 min before,5 min after surgery,and 1 hour after day 7 using the BL-420F biofunction system.The diagnostic ultrasound in-strument was used to examine the heart structure and function of each group of mice.The influence of myo-cardial histology was observed by hematoxylin and eo-sin (HE) staining and Sirius red(Sirius Red)stai-ning.The changes of creatine kinase isozymin-MB(CK-MB),cardiac troponin Ⅰ(cTnⅠ)and lactate de-hydrogenase(LDH)in mouse serum were assessed by ELISA kit.The expressions of PPARα signaling related proteins and apoptosis-related proteins were detected by immunofluorescence and Western blot.Results Compared with MI mice,Hyp could significantly im-prove the ECG abnormality of MI mice,increase the left ventricular ejection fraction(LVEF)and left ventricu-lar short axis shortening rate(LVFS),reduce serum cTnⅠ content,CK-MB and LDH activity,and reduce myocardial fibrosis,infarct area and cardiomyocyte ap-optosis in the MI area.Meanwhile,this research found that Hyp could activate PPARα signaling pathway and regulate apoptosis-related proteins.However,the car-dioprotective effect of hyperoside was reversed by the combination with the treatment of the PPARα signaling inhibitor,GW6471.Conclusions Hyp has an impro-ving effect on energy metabolism and apoptosis in mice after MI,and the mechanism may be related to its acti-vation of PPARα signaling pathway.
