Resveratrol attenuates hepatic inflammation and oxidative stress in rheumatoid arthritis via Nrf2/Keap1 pathway
- VernacularTitle:白藜芦醇通过Nrf2/Keap1途径减轻类风湿关节炎肝脏炎症和氧化应激
- Author:
Xue-fei FAN
1
;
Jian ZHOU
;
Su-huan CHEN
;
Meng-yan ZHANG
;
Hao-miao LIU
;
Rui SU
;
Guang-yi CHEN
;
Yu-bao SHAO
;
Tao YAO
;
Xiao-yu CHEN
Author Information
- Publication Type:Journal Article
- Keywords: rheumatoid arthritis; liver injury; resvera-trol; inflammation; oxidative stress; Nrf2/Keap1
- From: Chinese Pharmacological Bulletin 2025;41(5):861-867
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the therapeutic effects of resveratrol(Res)on hepatic inflammation and oxida-tive stress in rheumatoid arthritis(RA),and to eluci-date the relationship of the regulatory mechanism of the Nrf2/Keap1 signaling pathway in it.Methods A mouse model of arthritis was induced using chicken type Ⅱ collagen in combination with complete Freund's adjuvant,and Res was administered by tube feeding for treatment.Serum liver function indices and levels of hepatic inflammation and oxidative stress were detected in mice.An in vitro cellular model of hepatic inflam-mation and oxidative stress was established by treating mouse primary hepatocytes(MPHs)with TNF-α(5μg·L-1),cell proliferation inhibition was detected by CCK-8,and inflammation and oxidative stress-relat-ed indices were detected by protein blotting.The in-trinsic mechanisms by which Res attenuated hepatic in-flammation and oxidative stress in rheumatoid arthritis were explored by treating MPHs with Nrf2 inhibitor and Keap1 overexpression plasmid.Results Res signifi-cantly reduced the levels of inflammation and oxidative stress in hepatic tissues of collagen-induced arthritis mice as well as TNF-α-treated MPHs,and activated the Nrf2/Keap1 signaling pathway.Inflammation and oxidative stress levels in MPHs were exacerbated by the use of Nrf2 inhibitors and Keap1 overexpression,which promoted apoptosis.Conclusion Res attenuates he-patic inflammation and oxidative stress in rheumatoid arthritis via the Nrf2/Keap1 pathway.
