Exploring mechanism of action of hypericin in antidepressant effects based on single-cell sequencing
- VernacularTitle:基于单细胞测序探讨金丝桃素抗抑郁的作用机制
- Author:
Hui-xin NI
1
;
Hai-xin LIU
;
Bing-can ZHOU
;
Ming-heng CHEN
;
Ping-yan LIN
;
Zheng-tao GAO
;
Xin-pei LIN
;
Yao LIN
;
Fang-zhen WU
;
Qian XU
Author Information
- Publication Type:Journal Article
- Keywords: depression; single-cell sequencing; hyper-forin; cerebral perivascular macrophages; Ccl24; che-mokine signaling pathway
- From: Chinese Pharmacological Bulletin 2025;41(5):837-843
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the antidepressant mechanism of hyperforin via the utilization of single-cell sequencing technology.Methods C57BL/6 mice were randomly divided into the control group,depres-sion model group,and hyperforin intervention group.The chronic unpredictable mild stress(CUMS)model was induced and drug interventions were administered for 28 d.Behavioral experiments were conducted to as-sess depressive symptoms,and hippocampal tissue was collected for single-cell RNA sequencing.Key cell populations and differentially expressed genes across groups were identified,followed by PPI network,GO,and KEGG enrichment analysis.Results Behavioral experiments indicated that CUMS successfully induced depressive symptoms in mice,while hyperforin im-proved depressive behavior.In the depression model group,the proportion of brain perivascular macrophages(PVM)increased,and this proportion decreased after hyperforin intervention,approaching the level seen in the control group.The top 20 common differentially ex-pressed genes in the PVM subpopulation were Saa3,Hbb-bs and Ccl24.PPI network analysis identified core targets,including Ccl2,Dhx9,C3,Msr1,Cxcl2 and Cx3cr1.KEGG enrichment analysis revealed pathways related to chemokines,phagosome formation,and inosi-tol phosphate metabolism.Conclusion The antide-pressant mechanism of hyperforin may be related to the regulation of Ccl24 and its related chemokine signaling pathway by PVM.
