Advances in testicular organoid culture technology
10.3760/cma.j.cn101441-20240805-00285
- VernacularTitle:睾丸类器官培养技术的进展
- Author:
Minghao HUANG
1
;
Guoyao DU
;
Denglong WU
;
Wei LE
Author Information
1. 同济大学医学院，上海 200092
- Publication Type:Journal Article
- Keywords:
Testis;
Organoid;
Biological scaffold;
3D culture
- From:
Chinese Journal of Reproduction and Contraception
2025;45(1):96-102
- CountryChina
- Language:Chinese
-
Abstract:
With the increasing incidence of male reproductive system diseases and reproductive dysfunctions, diagnosis and treatment of male infertility and fertility preservation have become clinical challenges to be overcome. Organoids, a kind of cell product cultured in vitro under different microenvironments by using adult stem cells or embryonic stem cell, can possess histological anatomy and physiological function that are similar to natural tissues. In recent years, technological breakthroughs have been made in testicular organoids in terms of sperm proliferation and differentiation induced in vitro, providing a new idea for solving the above problems. This article briefly introduces common testicular organoid techniques (micropore culture, microfluidics culture method, three-layer gradient system, air-liquid interface culture, extracellular matrix hydrogel scaffold, hanging drop cultures, and 3D printing technology, etc.), including technical features, research conditions, technical bottlenecks and summarizes the application characteristics and situations of some ordinary organoid culture solutions in the field of testicular organoids as well.
