Analysis of immunological indicators in patients with unexplained recurrent pregnancy failure
10.3760/cma.j.cn101441-20240610-00211
- VernacularTitle:不明原因反复妊娠失败患者的免疫学指标分析
- Author:
Jing DONG
1
;
Haihui GAO
1
;
Liqin WANG
1
;
Lin PAN
1
;
Lei ZHAO
1
Author Information
1. 青岛大学附属医院风湿免疫科，青岛 266000
- Publication Type:Journal Article
- Keywords:
Recurrent miscarriage;
Repeated implantation failure;
Antinuclear antibodies;
Lymphocyte subsets;
Natural killer cells
- From:
Chinese Journal of Reproduction and Contraception
2024;44(11):1175-1179
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To classify and analyze the immunological indicators of patients with unexplained recurrent pregnancy failure, and explore whether the immunological indicators of these patients were related to pregnancy failure.Methods:We retrospectively studied 91 patients who visited the Rheumatology and Immunology Department of the Affiliated Hospital of Qingdao University from January 2022 to June 2022 due to recurrent pregnancy failures, including recurrent spontaneous abortion and recurrent implantation failures (RIF). We adopted case-control study method. Analysis was conducted from two different perspectives: antinuclear antibody (ANA) positivity and natural killer (NK) cell elevation. Among them, there were 34 ANA positive patients and 57 ANA negative patients; 32 patients had normal NK cells and 59 had elevated NK cell. Clinical data, duration of infertility, number of miscarriages, number of biochemical pregnancies, number of recurrent implant failures, and other information from patients were collected. Immunological indicators included ANA, anti-extractable nuclear antigen antibody, antiphospholipid antibodies, peripheral blood lymphocyte subsets, complement, immunoglobulin, rheumatism, etc. Routine laboratory tests included blood routine, urine routine, liver and kidney function, etc. The results were statistically analyzed.Results:There were no statistically significant differences between the ANA-positive and negative groups in terms of the number of spontaneous abortion, the number of post-transplantation abortions, the total number of pregnancy failures, and previous gynecological and chronic diseases (all P>0.05). The number of no-implanting after transplantation in the ANA-positive group was 3.20±2.04, which was significantly higher than that in the ANA-negative group (1.47±0.96, P=0.004). The proportion of CD19 +B cells [(12.96±4.26)%] and CD3 -HLA -DR + activated B/NK cells [(14.58±5.45)%] in the ANA negative group were significantly higher than those in the ANA positive group [(10.23±3.54)%, P=0.007; (11.34±4.11)%, P=0.009]. There were no significant differences in the number of spontaneous abortion, the number of fetal abortion after transplantation and the total number of pregnancy failure between the normal NK cell group and the increased NK cell group (all P>0.05). Conclusion:The number of RIF in ANA positive patients is significantly increased. The relationship between NK cells and pregnancy failure is not clear yet.
