Soil microbial characteristics in sheep sheds based on metagenomic second-generation sequencing
10.3760/cma.j.cn231583-20240914-00331
- VernacularTitle:基于宏基因组二代测序的羊舍土壤微生物特征研究
- Author:
Shaoxin AN
1
;
Yuanke YANG
;
Juntang ZHAO
;
Zeyu LYU
;
Yang GAO
;
Xiaokui GUO
;
Yongzhang ZHU
;
Yuanqing CAO
;
Jingyi LU
;
Yaoxia KANG
Author Information
1. 内蒙古医科大学公共卫生学院，呼和浩特 010000
- Publication Type:Journal Article
- Keywords:
Soil;
Microorganism;
Metagenomic second-generation sequencing
- From:
Chinese Journal of Endemiology
2025;44(4):286-291
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the characteristics of soil microbial composition in sheep sheds in agricultural and pastoral areas, and explore potential zoonotic pathogen species.Methods:Using cross-sectional survey method, soil samples from sheep sheds in the agricultural and pastoral areas of Darhan-Muminggan Joint County, Baotou City, Inner Mongolia Autonomous Region were collected from August to October 2023. Genomic DNA was extracted, and metagenomic second-generation sequencing was conducted. Microbial species annotation was carried out by Kaiju method, and the soil microbial composition and zoonotic pathogen species of sheep sheds in agricultural and pastoral areas were analyzed.Results:A total of 10 and 5 soil samples were collected from sheep sheds in agricultural and pastoral areas, respectively. The results of β diversity analysis showed that there was a significant separation trend in soil samples from sheep sheds in agricultural and pastoral areas, and the interpretation of principal co-ordinates analysis (PCoA) 1 and PCoA2 were 27.8% and 17.4%, respectively. There was no significant difference in the microbial composition of soil samples from sheep sheds in agricultural and pastoral areas ( R = 0.09, P = 0.242). At the phylum level, the dominant bacterial phyla were Actinobacteria, Proteobacteria, Firmicutes, and Bacteroidetes. At the genus level, the dominant bacterial genera were Corynebacterium, Luteimonas, Atopostipes, and Salinicoccus. A variety of zoonotic pathogens were detected, including Brucella melitensis, Mycobacterium tuberculosis, and Clostridium tetani in 15 soil samples. Conclusion:The soil microorganisms in sheep sheds in agricultural and pastoral areas are diverse, and zoonotic pathogens such as Brucella melitensis, Mycobacterium tuberculosis, and Clostridium tetani are detected.
