A method for determining fluoride in water using a fully automated biochemical analyzer
10.3760/cma.j.cn231583-20240402-00082
- VernacularTitle:全自动生化分析仪测定水中氟化物的方法研究
- Author:
Tingting LIU
1
;
Kaiqian WANG
1
;
Junhua ZHANG
1
;
Yue HU
1
;
Ji'an XIE
1
;
Gang DING
1
;
Weidong LI
1
;
Shudong XU
1
Author Information
1. 安徽省疾病预防控制中心理化检验室，合肥 230601
- Publication Type:Journal Article
- Keywords:
Fluoride;
Fully automatic biochemical analyzer;
Automatic detection;
Efficient
- From:
Chinese Journal of Endemiology
2025;44(2):142-145
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for determination of fluoride in water using a fully automated biochemical analyzer (abbreviated as this method).Methods:Based on the parameters of the fully automatic biochemical analyzer, appropriate reagent volumes and reaction time were optimized to determine fluoride ions in water within the standard curve range (0.0 - 2.0 mg/L). The method was validated through evaluation of linear range, detection limit, precision, accuracy, and comparative analysis with the manual measurement results of fluoride reagent spectrophotometric method specified in the "Standard Examination Methods for Drinking Water - Part 5: Inorganic Nonmetallic indices" (GB/T 5750.5-2023).Results:Within the range of 0.0 - 2.0 mg/L fluoride mass concentration, the absolute values of the correlation coefficients were > 0.999 0 and the detection limit of this method was 0.082 mg/L. The relative standard deviation for the determination of fluoride level in water samples with low, medium and high fluoride mass concentrations was 4.03%, 2.16% and 1.68%, respectively. The spiked recovery rates were 98.1%, 99.5%, and 100.2%, respectively. There was no statistically significant difference between the results obtained by this method and manual measurement ( t = 1.07, P = 0.295). Conclusion:This method exhibits high sensitivity, accuracy, and efficiency in detecting fluoride, enabling rapid sample detection through instrumental automation instead of manual operation.
