Isolation and application value of Yersinia enterocolitica phage strain in Yunnan Province
10.3760/cma.j.cn231583-20240516-00129
- VernacularTitle:云南省小肠结肠炎耶尔森菌噬菌体分离及应用价值研究
- Author:
Shaogui ZI
1
;
Chunpeng MAO
;
Pan LIU
;
Fengyi YANG
;
Haipeng ZHANG
;
Peng WANG
;
Youhong ZHONG
Author Information
1. 云南省地方病防治所 云南省自然疫源性疾病防控技术重点实验室，大理671000
- Publication Type:Journal Article
- Keywords:
Bacteriophages;
Yersinia enterocolitica;
Biological properties;
Taxonomy;
Genomes
- From:
Chinese Journal of Endemiology
2025;44(3):173-178
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To isolate Yersinia enterocolitica phage and study its biological and genomic characteristics, and explore its application value. Methods:Phages were isolated from cecum of Apodemus chevrieri in the plague focus of wild rodent in Lijiang employing Yersinia enterocolitica (CMCC52301) as the host strain. After purification, proliferation and concentration, the morphology was observed by transmission electron microscope, and the host spectrum of the phage was determined by double-layer plate method. Phage DNA was extracted and sequenced, and the raw sequences data were assembled and visually analyzed, also performed genome functional annotation, phage classification status, phylogenetic tree, genome collinearity analysis, etc. Results:One strain of Yersinia enterocolitica phage was isolated and named vB_Yen_YN301-151, which had a head and a contractile tail. The phage only lysed Yersinia enterocolitica, and could not lyse other tested strains. And at a lysis temperature of 37 ℃, more transparent plaques were observed compared to at a lysis temperature of 21 ℃. The gene structure of this phage was double-stranded DNA, with a genome length of 51 176 bp and a GC content of 46.96%, and 95 genes were predicted to encode 15 structural morphological related proteins, 9 proteins involved in replication and metabolism, 3 DNA assembly functional proteins, 1 phage lysis related protein, and 67 hypothetical proteins. It was identified that the phage belong to the Drexlerviridae family of the Caudoviricetes class, clustered with vB_YenM_534, vB_Yen_X1, vB_YenM_281, and vB_YenM_531 in the phylogenetic tree and exhibited good collinearity with PY100, vB_YenM_ 25, and vB_Yen_X1. Conclusions:The successfully isolated vB_Yen_YN301-151 is a novel phage belonging to the Drexlerviridae family, and its gene encoded proteins are mostly hypothetical proteins. It only lyses Yersinia enterocolitica, with host specificity and has the potential to be used as diagnostic phage for screening Yersinia enterocolitica.
