New methods for target identification of complex components in traditional Chinese medicine and research progress in their applications
- VernacularTitle:中药复杂成分靶点鉴定新方法及其应用研究进展
- Author:
Qian ZHANG
1
;
Jun-yu XU
;
Xiao-xi LU
;
Ji-gang WANG
;
Piao LUO
Author Information
- Publication Type:Journal Article
- Keywords: traditional chinese medicine; complex components; target identification; chemical proteomics; cell thermal shift as-say; modernization of traditional chinese medicine
- From: Chinese Pharmacological Bulletin 2025;41(11):2001-2008
- CountryChina
- Language:Chinese
- Abstract: This article presents a comprehensive review of new methods for target identification of complex components in tradi-tional Chinese medicine(TCM)and research progress in their applications.It systematically summarizes classical approaches and cutting-edge technical systems for target identification,cov-ering multiple strategies such as proteomic analysis,fluorescence resonance energy transfer(FRET)technology,network pharma-cology prediction models,high-throughput biochip screening,tar-get capture strategies based on molecular affinity fishing,gene site-directed mutagenesis verification,and co-crystallization structure analysis of target proteins.The review emphasizes the critical role of target identification in elucidating the action mechanisms of TCM and facilitating new drug development,lay-ing a foundation for promoting the modernization of TCM.
