The effect of LncRNA NNT-AS1 targeting miR-199a-5p on the proliferation,migration and invasion of oral squamous cell carcinoma cells
- VernacularTitle:LncRNA NNT-AS1靶向miR-199a-5p对口腔鳞癌细胞增殖、迁移和侵袭的影响
- Author:
Lanlan SHENG
1
;
Xiaofei OU
1
Author Information
- Publication Type:Journal Article
- Keywords: squamous cell carcinoma of head and neck; mouth neoplasms; RNA,long noncoding; long non-coding RNA NNT-AS1; microRNA-199a-5p
- From: Tianjin Medical Journal 2025;53(11):1131-1137
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effect of long non-coding RNA(LncRNA)NNT-AS1 on proliferation,migration and invasion of oral squamous cell carcinoma(OSCC)cells by regulating microRNA(miR)-199a-5p.Methods CAL-27 cells were assigned into the control group,the sh-NC group,the sh-NNT-AS1 group,the sh-NNT-AS1+miR-In-NC group and the sh-NNT-AS1+miR-199a-5p-In group.The proliferation,colony formation,migration and invasion abilities of CAL-27 cells were detected by EdU method,clone formation,scratch healing and Transwell experiments.The expressions of LncRNA NNT-AS1 and miR-199a-5p in human keratinocyte cell line HaCaT,human OSCC cell line CAL-27 and each group of cells were detected by qRT-PCR experiment.Western blot experiments were performed to detect the expressions of epithelial-mesenchymal transition(EMT)-related proteins[E-cadherin,N-cadherin,Vimentin,and epidermal growth factor receptor(EGFR)].The targeting relationship between LncRNA NNT-AS1 and miR-199a-5p was verified by dual luciferase reporter gene assay.Results Compared with HaCaT cells,the level of LncRNA NNT-AS1 was elevated in CAL-27 cells,while the level of miR-199a-5p was decreased(P＜0.05).Compared with the control group and the sh-NC group,the EdU positive rate,colony formation rate,scratch healing rate,cell invasion number,expression levels of LncRNA NNT-AS1,N-cadherin,Vimentin and EGFR proteins were decreased in the sh-NNT-AS1 group,while the expression levels of miR-199a-5p and E-cadherin protein were increased(P＜0.05).Compared with the sh-NNT-AS1 group and the sh-NNT-AS1+miR-In-NC group,the EdU positive rate,colony formation rate,scratch healing rate,cell invasion number,N-cadherin,Vimentin and EGFR protein expression levels were increased in the sh-NNT-AS1+miR-199a-5p-In group,while the expression levels of miR-199a-5p and E-cadherin protein were decreased(P＜0.05).Compared with the miR-NC group,the relative luciferase activity of NNT-AS1-WT cells transfected with miR-199a-5p mimic was decreased(P＜0.05).Conclusion LncRNA NNT-AS1 is highly expressed in OSCC.It can promote the proliferation,migration,invasion and EMT process of OSCC cells by negatively regulating miR-199a-5p.
