Effects and mechanisms of Shenwuyishen tablets in treating chronic kidney disease
- VernacularTitle:参乌益肾片治疗慢性肾脏病的药效及作用机制
- Author:
Jin-hong REN
1
;
Ze LIU
;
Xiang-da MENG
;
Zi-ling YUAN
;
Zhi-tang SUN
;
Cheng HAN
Author Information
- Publication Type:Journal Article
- Keywords: Shenwuyishen tablets; chronic kidney disease; quercetin; FOS; JUN
- From: Chinese Pharmacological Bulletin 2025;41(11):2185-2193
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the therapeutic effect of Shenwuyishen tablets(SWYST)in treating chronic kidney disease(CKD)and the underlying mechanism.Methods The pharmacological action of SWYST was evaluated in folic acid(FA)-induced mouse models by levels of serum creatinine and blood urea nitrogen,in-dexes of tubulointerstitial inflammation and tubular in-jury,and degrees of renal fibrosis.Differential genes from 22 types of cells in kidney tissue of CKD were i-dentified by single-cell and single-nuclei RNA sequen-cing datasets.Network pharmacology was used to pre-dict key ingredients,cells,and targets.The binding mode between the key ingredients and key targets was elucidated using molecular docking and molecular dy-namics simulation.Results Serum creatinine and blood urea nitrogen levels,tubulointerstitial inflamma-tion and tubular injury indexes,and renal fibrosis de-grees were significantly reduced by SWYST administra-tion.Quercetin,kaempferol,luteolin,baicalein and wogonin were considered as key components that main-ly acted through FOS and JUN of endothelial cells,neu-trophils and thick ascending limb cells to ameliorate CKD.Molecular docking and molecular dynamics sim-ulation revealed that quercetin stably occupied the cross pocket of FOS-JUN heterodimers to inhibit the binding between FOS-JUN heterodimers and DNA.Conclusion SWYST inhibits the binding of FOS-JUN heterodimers and DNA of endothelial cells,neutrophils and thick ascending limb cells to ameliorate CKD.
