RICH1 regulates myocardial fibrosis through TGF-β/SMAD signaling pathway
- VernacularTitle:RICH1通过TGF-β/SMAD信号途径调控心肌纤维化
- Author:
Lu-xuan WAN
1
;
Ying-qing HU
;
Yuan-yuan LIU
;
Yong-song TANG
;
Jun-yi HUANG
;
Zi-xuan ZHANG
;
Xiao-xiao MAO
;
Xin-wen NIE
;
Zhan-hong REN
Author Information
- Publication Type:Journal Article
- Keywords: RICH1; myocardial fibrosis; TGF-β1; MCFs; SMAD2/3; cardiac failure
- From: Chinese Pharmacological Bulletin 2025;41(11):2089-2096
- CountryChina
- Language:Chinese
- Abstract: Aim To reveal the mechanism of CIP4 homologs protein 1(RICH1)are involved in the regu-lation of myocardial fibrosis.Methods Mouse cardiac fibroblasts(MCFs)cells were treated with transforming growth factor-β(TGF-β1)to induce the formation of a myocardial fibrosis cell model;the level of the target protein was detected by Western blotting;and the RICH1 gene was detected by transfection of the cells with plasmid.The RICH1 gene was overexpressed(RICH 1 OE)using plasmid transfection;the RICH1 gene was silenced using siRNA fragment(siRICH1);and the expression levels of myocardial fibrosis marker genes,such as Col1 a1,Col3 a1,and Acta2,were de-tected using RT-qPCR.Results RICH1 was signifi-cantly down-regulated in TGF-β1-treated MCFs;the expression levels of myocardial fibrosis marker genes,such as Col1 a1,Col3a1,and Acta2,were down-regu-lated in the RICH1 OE+TGF-β1 group;and in the siRICH1+TGF-β1 group,myocardial fibrosis marker genes,such as Col1 a1,Col3a1 and Acta2 were up-regulated at the expression level;phosphorylated SMAD2(p-SMAD2)and phosphorylated SMAD3(p-SMAD3)levels were down-regulated in the siRICH1 OE+TGF-β1 group.p-SMAD2 and P-SMAD3 levels were upregulated in the siRICH1+TGF-β1 group.Conclusion RICH1 inhibits TGF-β1-induced myo-cardial fibrosis;RICH1 inhibits TGF-β1-induced myo-cardial fibrosis by negatively regulating the SMAD2/3 signaling pathway.
