Effect of total flavonoids of Chuzhou chrysanthemum on activity and migration of rat brain artery endothelial cells induced by hypoxia/reoxygenation injury and its relationship with VEGFR2
- VernacularTitle:滁菊总黄酮对缺氧/复氧损伤致大鼠脑血管内皮细胞活性、迁移的影响及与VEGFR2的关系
- Author:
Xing-yu ZHANG
1
;
Xiao WANG
;
Miao WU
;
Shuo CHEN
;
Zhi-wu CHEN
Author Information
- Publication Type:Journal Article
- Keywords: total flavonoids of Chuzhou chrysanthe-mum; endothelial cells; VEGFR2; hypoxia/reoxygen-ation injury; activity; migration
- From: Chinese Pharmacological Bulletin 2025;41(11):2075-2081
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effects of total fla-vonoids of Chuzhou chrysanthemum(TFCC)on the activity and migration of rat brain artery endothelial cells under hypoxia/reoxygenation(H/R)injury,as well as the relationship between TFCC's effects and vascular endothelial growth factor receptor 2(VEGFR2).Methods Primary rat brain artery endo-thelial cells were cultured,the H/R model of cells was constructed,VEGFR2 inhibitor SU5416(10 μmol·L-1)was used and a cell transfection model was estab-lished.The cell activity was detected by CCK-8,and the cell migration was detected by scratch method and Transwell assay.Results TFCC(30,90,270 mg·L-1)significantly increased the activity and migration ability of rat brain artery endothelial cells with H/R in-jury,the use of VEGFR2 blocker SU5416 and transfec-tion of VEGFR2 siRNA could significantly inhibit the enhanced activity and migration ability of TFCC on cer-ebral vascular endothelial cells in rats with H/R inju-ry.Conclusions TFCC promotes the activity and mi-gration ability of rat brain artery endothelial cell with H/R injury,and its mechanism is related to VEGFR2.
