MST1 knockdown attenuates the anti-renal fibrosis effect of pERK inhibition by salvianolic acid B
- VernacularTitle:MST1敲除减弱丹酚酸B抑制pERK抗肾纤维化作用
- Author:
Yu-xuan LI
1
;
Ying-ying WANG
;
Hui-min TAO
;
Jie-mei JIANG
;
Yan YANG
Author Information
- Publication Type:Journal Article
- Keywords: salvianolic acid B; HK-2; renal fibrosis; MST1; knockout; MAPK/ERK
- From: Chinese Pharmacological Bulletin 2025;41(11):2046-2052
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the MST1 knockdown at-tenuates the inhibitory effect of salvianolic acid B(Sal B)on pERK anti-renal fibrosis.Methods In vitro experiments stimulated human renal cortical proximal tubular epithelial cells(HK-2)with XMU-XP-1(10μmol·L-1)inhibitor;in vivo experiments induced wild-type(WT)and MST1 systemic knockout mouse(MST1-/-)renal fibrosis models with DEN/CCl4/C2H5OH(DCC)and interfered with Sal B(15 mg·kg-1·d-1,ig)intervention.Renal histopathology was observed by HE and Masson staining;α-SMA and pERK protein expression was detected by Western blot;and pERK protein expression was detected by im-munofluorescence.Results In vitro results showed that Sal B could inhibit pERK protein expression.In vivo results showed that Sal B could improve DCC-in-duced renal tissue pathological injury and inhibit α-SMA and pERK protein expression;MST1-/-aggrava-ted pathological injury and significantly up-regulated α-SMA and pERK protein expression.Conclusion Sal B reduces the stimulatory effect of XMU-MP-1 on HK-2 cells by inhibiting the phosphorylation of ERK protein;Sal B exerts its anti-renal fibrosis effect by inhibiting the phosphorylation of α-SMA protein and ERK pro-tein;and MST1-/-,aggravating the pathological inju-ry of renal tissue,significantly up-regulated the expres-sion of α-SMA and pERK protein,which in turn atten-uated the antirenal fibrosis effect of Sal B.
