The correlation between serum miR-21-5p,miR-1224-5p,miR-181a-5p expression with postoperative delayed healing in patients with femoral neck fracture
10.3969/j.issn.1005-6483.20241508
- VernacularTitle:股骨颈骨折病人血清微小RNA-21-5p、微小RNA-1224-5p、微小RNA-181a-5p表达与术后愈合延迟的相关性
- Author:
Jian GUO
1
;
Yuechao GUO
;
Zhe WANG
;
Wen AN
;
Yujian ZHANG
Author Information
1. 066000 河北秦皇岛,秦皇岛市第一医院骨科
- Publication Type:Journal Article
- Keywords:
femoral neck fracture;
miR-21-5p;
miR-1224-5p;
miR-181a-5p;
delayed healing
- From:
Journal of Clinical Surgery
2025;33(10):1105-1108
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between the expression of serum microRNA-21-5p(miR-21-5p),microRNA-1224-5p(miR-1224-5p),and microRNA-181a-5p(miR-181a-5p)with postoperative delayed healing in patients with femoral neck fracture(FNF).Methods From October 2021 to April 2024,371 patients with FNF admitted to our hospital were included as subjects.The patients were assigned into a study group(delayed healing,68 cases)and a control group(normal healing,303 cases)based on whether the fracture ends healed 4 months after surgery.Real-time fluorescence quantitative PCR(qRT-PCR)was applied to detect the expression levels of serum miR-21-5p,miR-1224-5p,and miR-181a-5p.Multivariate Logistic regression was applied to analyze the influencing factors of postoperative healing delay in FNF patients.ROC curve was applied to analyze the predictive value of serum miR-21-5p,miR-1224-5p,and miR-181a-5p for postoperative healing delay in FNF patients.Results The levels of serum miR-21-5p,miR-1224-5p,and miR-181a-5p in the study group were lower than those in the control group(P＜0.05).Multivariate Logistic analysis showed that age ≥ 60 years old,diabetes,and smoking were risk factors for postoperative delayed healing in FNF patients(P＜0.05),and miR-21-5p,miR-1224-5p and miR-181a-5p were protective factors for postoperative delayed healing in FNF patients(P＜0.05).ROC curve showed that the AUC values of miR-21-5p,miR-1224-5p,and miR-181a-5p for predicting postoperative healing delay in FNF patients were 0.845,0.809,and 0.797,respectively.When the three were combined for prediction,the AUC increased to 0.933.The combined detection effect of the three indicators was better than that of any individual indicator(Zcombination vs.miR-21-5p=3.444,Zcombination vs.miR-1224-5p=4.401,Zcombination vs.miR-181a-5p=4.279,P＜0.05).Conclusion The levels of serum miR-21-5p,miR-1224-5p,and miR-181a-5p are reduced in FNF patients with postoperative delayed healing.The combined detection of the three may have a certain predictive value for postoperative delayed healing in FNF patients.
