Predictive value of umbilical artery ultrasound blood flow parameters combined with serum miR-148a and miR-424-5p levels for perinatal outcomes in pregnant women with preeclampsia
10.13431/j.cnki.immunol.j.20250083
- VernacularTitle:脐动脉超声血流参数联合血清miR-148a、miR-424-5p表达水平对子痫前期孕妇围产儿结局的预测价值
- Author:
Yu ZHANG
1
;
Xihui YANG
;
Yu WANG
Author Information
1. 新疆维吾尔自治区人民医院妇产超声科,乌鲁木齐 830000
- Publication Type:Journal Article
- Keywords:
preeclampsia;
ultrasound blood flow parameters;
miR-148a;
miR-424-5p;
perinatal outcomes
- From:
Immunological Journal
2025;41(8):582-589
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the predictive value of umbilical artery ultrasound blood flow parameters combined with the levels of serum miR-148a and miR-424-5p in pregnant women with preeclampsia for perinatal outcomes.Methods A total of 95 pregnant women with preeclampsia admitted from June 2020 to July 2023 were enrolled as the research group and divided into the poor outcome group(n=37)and the good outcome group(n=58)according to the perinatal outcomes.In addition,152 healthy pregnant women who underwent prenatal examinations during the same period were included as the control group.Color Doppler ultrasound examination was performed to determine umbilical artery blood flow parameters:resistance index(RI),pulpability index(PI),and the ratio of maximum systolic blood flow velocity to diastolic blood flow velocity(S/D).The relative expression levels of serum miR-148a and miR-424-5p were detected by real-time fluorescence quantitative PCR(RT-qPCR).Multivariate logistic regression was used to analyze the influencing factors of poor perinatal outcomes in pregnant women with preeclampsia.The receiver operating characteristic(ROC)curve was drawn to analyze the predictive value of umbilical artery RI,PI,S/D and serum miR-148a and miR-424-5p for perinatal outcomes in pregnant women with preeclampsia.Results Compared with the control group,the expression levels of umbilical artery RI,PI,S/D and serum miR-424-5p in the research group all increased,while the expression level of serum miR148a decreased(P＜0.01).The proportions of perinatal infants with preterm birth,asphyxia,intrauterine growth restriction,low birth weight and the incidence of poor outcomes in the research group were all higher than those in the control group(P＜0.05).Compared with the good outcome group,the expression levels of umbilical artery RI,PI,S/D and serum miR-424-5p in the poor outcome group all increased,while the expression level of serum miR-148a decreased(P＜0.01).Umbilical artery RI,PI,S/D and serum miR-424-5p were all risk factors for poor perinatal outcomes,while serum miR-148a was a protective factor(P＜0.01).The area under the curve(AUC)of umbilical artery RI,PI,S/D combined with serum miR-148a and miR-424-5p for predicting poor perinatal outcomes in pregnant women with preeclampsia was significantly greater than that of RI(Z=3.489,P＜0.001),PI(Z=3.089,P＜0.001),and S/D(Z=3.296,P=0.001),miR-148a(Z=3.542,P＜0.001)and miR424-5p(Z=2.916,P=0.004)for prediction alone,and was also greater than that predicted by the combination of ultrasound blood flow parameters(RI,PI,S/D)(Z=2.457,P=0.014),and by the combination of serum miR-148a and miR-424-5p(Z=2.214,P=0.027).Conclusion The expression levels of umbilical artery RI,PI,S/D and serum miR-424-5p in pregnant women with preeclampsia are all increased,while the expression level of serum miR-148a is decreased,which is associated with poor perinatal outcomes.The combination of the above indicators has certain evaluation value for the perinatal outcomes of pregnant women with preeclampsia.
