Effect and Mechanism of Vitamin D on Tfh Cells in MRL/lpr Lupus Mice
10.3870/j.issn.1004-0781.2025.10.001
- VernacularTitle:维生素D对MRL/lpr狼疮小鼠Tfh细胞的影响及其机制
- Author:
Xiaoting HOU
1
;
Lin LI
;
Fang YUAN
;
Cui SHAO
;
Taiyong YIN
;
Dongsheng DING
;
Hongtao XU
;
Minshu ZOU
Author Information
1. 武汉科技大学医学院,武汉 430065
- Publication Type:Journal Article
- Keywords:
Vitamin D;
Systemic lupus erythematosus;
Follicular helper T cells;
Ca-CaN-NFAT
- From:
Herald of Medicine
2025;44(10):1525-1530
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect and molecular mechanism of vitamin D(VitD)on Tfh cells of MRL/lpr lupus mice.Methods C57/B6 mice and MRL/lpr lupus mice were transfected with siRNA to construct VDR knockout mouse models.Splenic Tfh cells of C57/B6 mice and MRL/lpr lupus mice were divided into control group,lupus group and VDRsiRNA lupus group(treated with vitamin D 0,1 and 10 nmol·L-1,respectively)by siRNA transfection.The percentage of Tfh cells was detected by flow cytometry.MRL/lpr lupus mice Peyer node Tfh cells were randomly divided into 7 groups,blank control group,vitamin D dose groups of 1 and 10 nmol·L-1,paricalcitol group(VitD 10 nmol·L-1+PA),VDRsiRNA control group,VDRsiRNA group(VitD 10 nmol·L-1),CaN inhibitor group(VitD 10 nmol·L-1+CsA),and incubated for 72 h.The concentration of calcium ions in Tfh of each group was detected.The expressions of AT1R,NFAT,CaN and P-CaN in Tfh cells were determined by Western blotting.Results The percentage of Tfh cells decreased significantly with the increase of vitamin D dose.Vitamin D CaN reduce the intracellular calcium concentration of Tfh,up-regulate the expression of AT1 protein in Tfh cells,and down-regulate the expression of CAN,P-CaN and NFAT protein in a dose-dependent manner,and the effect is more obvious when combined with PA.Conclusion Vitamin D may regulate the activation of follicular T helper cells in MRL/lpr mice via the Ca-CaN-NFAT pathway.
