EIF5A2 promotes epithelial mesenchymal transition in intrahepatic chol-angiocarcinoma cells through the PI3K/AKT signaling pathway
10.3969/j.issn.1009-9905.2025.10.001
- VernacularTitle:EIF5A2通过PI3K/AKT信号通路促进肝内胆管癌细胞上皮间质转化
- Author:
Shao-hua YANG
1
;
Yong-ping XU
;
Zhuo-yu ZHAO
;
Shi-bo ZHANG
;
Xing-bao FANG
;
Zhou-jun LIAO
Author Information
1. 云南省曲靖市第一人民医院 肝胆胰外科(云南 曲靖 655000)
- Publication Type:Journal Article
- Keywords:
Intrahepatic cholangiocarcinoma;
Eukaryotic translation initiation factor 5A2;
Migration;
Invasion;
PI3K/AKT signaling pathway;
Epithelial-mesenchymal
- From:
Chinese Journal of Current Advances in General Surgery
2025;28(10):757-762
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the the differential expression of EIF5A2 in intrahepatic cholangiocarcinoma cell lines RBE,HCCC9810,and HUCCT1,and its effects on HCCC9810 cell migration and invasion,epithelial mesenchymal transition,and PI3K/AKT signaling pathway.Methods:The differential expression of EIF5A2 in RBE,HCCC9810,and HUCCT1 cell lines was detected using WB method.The HCCC9810 cell line,with the highest expression of EIF5A2,was selected for this experiment.The expression of EIF5A2 in HCCC9810 cell line was silenced by transient transfection of small interfering RNA.The best silencing effect of small interfering RNA was screened by WB.Scratch assay and Tran-swell migration invasion assay were used to detect the effect of silencing EIF5A2 on the migration and invasion ability of HCCC9810 cells.WB was used to detect the effect of silencing EIF5A2 on PI3K/AKT signaling pathway and epithelial mesenchymal transition in HCCC9810 cells.Results:The WB results showed that EIF5A2 had the highest expression in the HCCC9810 cell line,and siRNA1 had the best silencing effect on EIF5A2 in the HCCC9810 cell line.Scratch assay and Transwell migration invasion assay results showed that silencing EIF5A2 in the HCCC9810 cell line resulted in a decrease in cell invasion and metastasis ability(P＜0.05).At the same time,the expression of p-PI3K and p-AKT in the PI3K/AKT signaling pathway was significantly decreased(P＜0.05),while the epithelial cell marker E-cadherin expression increased(P＜0.05)and the stromal cell marker N-cadherin expression decreased(P＜0.05).Conclusion:EIF5A2 may promote epi-thelial mesenchymal transition and enhance the migration and invasion ability of intrahepatic cholangiocarcinoma cells through the PI3K/AKT signaling pathway.
