TGF-β1 expression regulated by lncRNA H19 and its effect on the function of human ovarian granulosa cells
10.3760/cma.j.cn101441-20220602-00245
- VernacularTitle:lncRNA H19调控TGF-β1的表达及对人卵巢颗粒细胞功能的影响
- Author:
Xiuhong SUN
1
;
Junhui WAN
;
Shuang QIN
;
Xiangcai WEI
Author Information
1. 广州市妇女儿童医疗中心超声科，广州 510600
- Publication Type:Journal Article
- Keywords:
Polycystic ovary syndrome;
Transforming growth factor-β1;
Long noncoding RNA H19;
Granulosa cell
- From:
Chinese Journal of Reproduction and Contraception
2023;43(9):926-931
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship between lncRNA H19 gene and transforming growth factor (TGF)-β1 in polycystic ovary syndrome (PCOS) granulosa cells and their effects on the proliferation and apoptosis of granulosa cells. Methods:Human ovarian granulosa cell line KGN was treated with TGF-β1 of 2 μg/L, 4 μg/L, and 10 μg/L, and the expression level of H19 was detected by Q-PCR method, and the H19 overexpression plasmid pcDNA3.0-H19 and siH19-1617 were transfected into KGN, the expression of TGF-β1 was detected by Western blotting, the proliferation of granulosa cells was detected by CCK8 method, and the apoptosis of granulosa cells was detected by flow cytometry.Results:After adding TGF-β1, the expression of H19 increased compared with untreated cells. When the concentration of TGF-β1≥4 μg/L, the expression of H19 increased significantly, and the difference was statistically significant ( P=0.023). After H19 was overexpressed, the expression of TGF-β1 was significantly higher than that of control group KGN+pcDNA3.0-NC transfected with empty plasmid ( P=0.017), and the expression of TGF-β1 after H19 silencing was significantly lower than that of control group KGN+NC ( P<0.001). After H19 overexpression, the proliferation ability of KGN cells was enhanced at 48 h and 72 h, but the apoptosis ability was weakened. After H19 was silenced, the proliferation ability of KGN cells was weakened at 48 h and 72 h, but the apoptosis ability was enhanced. Conclusion:H19 can regulate the expression of downstream protein TGF-β1, promote the proliferation of PCOS ovarian granulosa cells, and inhibit cell apoptosis.
