Forensic detection of processed pork products based on STR markers
10.13618/j.issn.1001-5728.2025.04.007
- VernacularTitle:基于STR的猪肉深加工食品法医检测研究
- Author:
Bo YANG
1
;
Qiong JIA
;
Wenhua MA
;
Jun HE
;
Lina JIA
;
Hao NIE
;
Zhiguang WANG
;
Jian YE
;
Fan YANG
;
Xingchun ZHAO
Author Information
1. 山西医科大学,山西太原 030001
- Publication Type:Journal Article
- Keywords:
Forensic biological evidence;
Pig STR multiplex amplification system;
STR analysis;
Processed pork products;
DNA degradation
- From:
Chinese Journal of Forensic Medicine
2025;40(4):423-427,433
- CountryChina
- Language:Chinese
-
Abstract:
Objective To verify the applicability of a pig STR multiplex amplification system for detecting processed foods containing pork and their digestive samples,and to evaluate its potential in food safety and forensic biological evidence analysis.Methods DNA profiles were obtained using the pig STR amplification system from food samples with different levels of processing(raw pork,boiled pork,fried pork,and sausage)and from digestive samples(rat gastric contents).The influence of processing methods on DNA integrity was assessed.The uniformity of large-scale processed ham products,the consistency of DNA profiles from different parts of the same sample,and the DNA degradation patterns after rat digestion were examined.Results STR profiling of pig DNA was successful in all tested samples.Short fragments showed high amplification stability,while long fragment signals weakened with increasing processing complexity.In processed ham products,DNA profiles were consistent across all sampled parts,with fragment drift within±0.5 bp.Analysis of rat gastric contents showed slight DNA degradation within 2 hours;after 3 hours,long fragment signals weakened,and after 4 hours,some loci signals were lost.Conclusion The pig STR multiplex amplification system exhibits excellent performance in detecting processed pork products and their digestive samples.It can meet the requirements of food traceability and forensic biological evidence analysis for processed pork,providing new insights for the advancement of forensic testing techniques in this field.
