Application of In-fusion Cloning Technology as a Teaching Example in the Molecular Biology Laboratory(A National First-class Undergraduate Course)
10.13865/j.cnki.cjbmb.2025.05.1009
- VernacularTitle:无缝克隆技术在国家级一流本科课程《分子生物学实验》中的应用实例
- Author:
Jing LUO
1
;
Ya-Nan LI
;
Yi-Di WANG
;
Dong YANG
;
Li TONG
;
Hong-Yu SHEN
;
Yan-Xia YIN
Author Information
1. 北京师范大学生命科学学院,北京市基因工程药物及生物技术重点实验室,北京 100875
- Publication Type:Journal Article
- Keywords:
in-fusion cloning;
restriction cloning;
alkaline phosphatase gene;
mutant;
National First-Class Course
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(10):1552-1558
- CountryChina
- Language:Chinese
-
Abstract:
In-fusion cloning technology,as a revolutionary and efficient molecular biology tool,has been applied in multiple research fields such as basic biology,biotechnology,and biomedicine.In this article,we introduce a teaching reform project suitable for undergraduate students in the course of"Molecular Bi-ology Laboratory",which utilizes in-Fusion cloning technology to construct a prokaryotic expression vector for alkaline phosphatase mutant genes.Through specific teaching cases,we systematically explored the design and implementation of experimental projects,and focused on analyzing the key and difficult points of the teaching content.Our teaching practice has found that the implementation of this educational re-form project has achieved very good results in enhancing students' core biological literacy,bioinformatics skills,research thinking,and innovation abilities.At the same time,the application of this technology can significantly improve the quality of experimental teaching,providing new ideas and practical refer-ences for promoting the reform and innovation of National First-Class Courses.
