Development of 96-channel genetic analyzer and its application in forensic medicine
10.13618/j.issn.1001-5728.2025.04.014
- VernacularTitle:96通道遗传分析仪研制及其在法医学上的应用
- Author:
Hailong CHANG
1
;
Dan SUN
1
;
Hua GUAN
1
;
Tao ZHANG
1
;
Erhui JIA
1
Author Information
1. 公安部第一研究所,北京 102200
- Publication Type:Journal Article
- Keywords:
High throughput;
STR detection;
Resolution;
Sensitivity;
Forensic medicine
- From:
Chinese Journal of Forensic Medicine
2025;40(4):463-467
- CountryChina
- Language:Chinese
-
Abstract:
This study introduces the development of a 96-channel genetic analyzer and its application in forensic medicine.The instrument integrates a fluorescence excitation system,a fluorescence spectral imaging system,and a data acquisition and analysis system,enabling the simultaneous detection of 96 DNA samples.Performance validation in forensic science demonstrated that the analyzer can resolve DNA fragments differing by 1 bp,with accurate DNA typing and a detection sensitivity of 0.125 ng,while maintaining good repeatability.The system can effectively process various forensic samples,including blood cards,saliva cards,and shed cell swabs.In practical applications,the analyzer has significantly improved detection efficiency and shown excellent performance.Looking forward,by broadening the detection range of the nine-color fluorescence spectrum and enhancing the level of data intelligence,the instrument is expected to further strengthen its high-throughput multi-locus detection capability and efficiency,providing more powerful technical support for forensic DNA analysis.
