Screen of Disulfidptosis-related Colorectal Cancer Diagnostic and Therapeutic Target:Integrated Single-cell and Bulk RNA Sequencing Data
10.13865/j.cnki.cjbmb.2025.09.1203
- VernacularTitle:基于单细胞和转录物组测序筛选结直肠癌双硫死亡相关诊疗靶标
- Author:
Yang YANG
1
;
Yi-Xuan MA
;
Xin-Yue FAN
;
Wen-Xue ZHAO
;
Yi-Ming QI
;
Ning GAO
;
Ju-Mei ZHAO
;
Juan DU
Author Information
1. 延安大学延安医学院基础医学研究实验中心,陕西延安 716000
- Publication Type:Journal Article
- Keywords:
ubiquinol-cytochrome c reductase core protein 1(UQCRC1);
disulfidptosis;
colorectal cancer(CRC)
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(10):1529-1540
- CountryChina
- Language:Chinese
-
Abstract:
Inflammatory response,immunosuppression,and drug sensitivity have been reported to have a significant correlation with the disulfidptosis levels in cancer patients.However,the value of disulfidpto-sis in colorectal cancer therapy remains unclear.Therefore,we classified the CRC cells into different cell types using single-cell sequencing data and cell-specific markers and analyzed their relationship with the cell disulfidptosis level.We found that the high disulfidptosis regions were concentrated in epithelial-like CRC cells.Further exploration using the disulfidptosis and programmed cell death 1 inhibitor therapy treated differential expression genes indicated that CRC patients with high disulfidptosis levels exhibited a lower risk profile and increased sensitivity to immunotherapy.By using the spatial transcriptomic analy-sis,we found that ubiquinol-cytochrome c reductase core protein 1(UQCRC1),a disulfidptosis-related gene,is highly expressed in epithelial-like CRC cells and co-localized with immune-infiltrated tumor re-gions.Additional bioinformatic analyses and experimental validation further confirmed that UQCRC1 was downregulated in CRC tissues.Overexpression of UQCRC1 suppressed CRC cell proliferation and migra-tion.These findings indicate that UQCRC1 is a potential target for CRC diagnosis and treatment.
