Intestinal microbiota in mice with nitrous oxide poisoning:A study based on 16S rDNA and metabolomics
10.13618/j.issn.1001-5728.2025.04.010
- VernacularTitle:基于16S rDNA和代谢组学技术的笑气中毒小鼠肠道微生物研究
- Author:
Congying LIU
1
;
Fenglin ZHANG
;
Yaoguang LI
;
Wen ZHANG
;
Keming YUN
;
Jiangwei YAN
;
Juan JIA
Author Information
1. 山西医科大学,山西晋中 030600;法庭毒物分析公安部重点实验室,山西晋中 030600;法医学山西省重点实验室,山西晋中 030600;山西省法医鉴识工程研究中心,山西晋中 030600
- Publication Type:Journal Article
- Keywords:
Nitrous oxide poisoning;
16S rDNA;
Metabolomics;
Differential microbiota;
Differential metabolites
- From:
Chinese Journal of Forensic Medicine
2025;40(4):438-443,449
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze changes in intestinal microbiota composition and metabolites in mice with nitrous oxide poisoning using 16S rDNA sequencing and metabolomics,and to examine correlations between gut microbes and metabolites in order to explore the mechanisms of nitrous oxide poisoning.Methods C57BL/6 mice were randomly divided into a control group and a nitrous oxide poisoning group(n=6).The poisoning group was exposed to 90,000 ppm nitrous oxide twice daily for 1 h over 28 days,while the control group was exposed to air.Fecal samples were collected 24 h after the last exposure.16S rDNA sequencing was used to analyze structural differences in microbial communities and identify significantly different taxa.Metabolomics analysis was performed to detect changes in fecal metabolites and identify differential metabolites.Correlation analysis was conducted between differential microbiota and metabolites.Results 16S rDNA sequencing showed that the poisoning group had increased microbial abundance compared with controls,while species diversity remained unchanged.Significant differences were observed in gut microbiota structure between groups.Metabolomics identified 112 differential metabolites related to nitrous oxide poisoning,mainly involving the cAMP signaling pathway and sphingolipid metabolism.Spearman correlation analysis revealed a strong association between differential microbiota and differential metabolites.Conclusion Nitrous oxide poisoning alters the structure and metabolic profiles of intestinal microbiota.Changes in microbial abundance affect multiple metabolic pathways,which may be related to damage to the nervous and hematological systems.These findings provide a basis for further research on the mechanisms of nitrous oxide poisoning and for clinical treatment.
