Preparation and Identification of a Novel Polypeptide Promoting Wound Healing
10.13865/j.cnki.cjbmb.2025.09.1327
- VernacularTitle:一种新型促伤口愈合的多肽制备及鉴定
- Author:
Yue-Xia ZHU
1
;
Biao HUANG
1
;
Yu-Bin LIAO
1
;
Chen-Xin WANG
1
;
Zhong-Hua LIU
1
;
Qiong LIAO
1
Author Information
1. 湖南师范大学生命科学学院生物化学与分子生物学系,长沙 410000
- Publication Type:Journal Article
- Keywords:
Odorrana tormota;
polypeptide;
cell proliferation;
wound healing
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(10):1402-1409
- CountryChina
- Language:Chinese
-
Abstract:
Wound repair and infection have long posed significant challenges in clinical medicine.Am-phibians,adapting to complex environments through long-term natural selection,have evolved skin sys-tems with remarkable abilities to resist external damage and promote rapid wound healing,making them an important source for discovering candidate molecules for wound healing.In this study,a novel peptide composed of 22 amino acids,with the sequence"VGKAGLETAACKATNSCFNIDW"and a molecular weight of 2299.6 D,was screened from the cDNA library of Odorrana tormota and named PN-VW22.The peptide was synthesized by solid-phase synthesis,and its effects on cell proliferation were evaluated using the CCK-8 assay and scratch wound healing assay in human keratinocytes(HaCaT)and mouse em-bryonic fibroblasts(NIH3T3).Antibacterial activity was assessed by determining the minimum inhibitory concentration(MIC).Results showed that PN-VW22 at various concentrations had no significant effect on the cell viability in NIH3T3 cells(P＞0.05),but significantly enhanced HaCaT cell viability at 0.5μmol/L(P＜0.001).Meanwhile,PN-VW22 induced cell proliferation and promoted wound healing in HaCaT cells,with a healing rate of 64.44％after 24 h incubation at 0.5 μmol/L.Furthermore,PN-VW22 exhibited antibacterial activity against Staphylococcus aureus with an MIC value of 13.92 μmol/L.In sum,this study identified PN-VW22 as a novel bifunctional peptide with both wound repair and anti-infective properties,providing a new toxin peptide template for the development of wound healing drugs.
