Genetic evolution analysis of chicken-origin H3N8 subtype avian influen-za virus and study on its pathogenicity to hamsters
10.3969/j.issn.1000-4718.2025.09.023
- VernacularTitle:一株鸡源H3N8亚型禽流感病毒的遗传进化和对仓鼠致病性的分析
- Author:
Ting LI
1
;
Mengyao WANG
;
Fangfang QIAO
;
Liji ZHANG
;
Wenjing YANG
;
Yuxin ZHANG
;
Jiangwu HUANG
;
Wanting ZHOU
;
Minhua SUN
;
Jun HE
;
Ming LIAO
Author Information
1. 华南农业大学兽医学院,人兽共患病防控制剂国家地方联合工程实验室,广东 广州 510642;广东省农业科学院动物卫生研究所,农业农村部禽流感等家禽重大疾病防控重点实验室,广东省畜禽疫病防治研究重点实验室,广东 广州 510640
- Publication Type:Journal Article
- Keywords:
avian influenza virus;
H3N8 subtype;
genetic evolution;
hamster pathogenicity
- From:
Chinese Journal of Pathophysiology
2025;41(9):1862-1872
- CountryChina
- Language:Chinese
-
Abstract:
AIM:A strain was isolated and identified as the H3N8 subtype of the avian influenza virus from a sick chicken at a farm in Yangjiang,Guangdong Province,named A/chicken/Yangjiang/552/2023(abbreviated as YJ/552).The aim of this research is to determine its genetic evolution,biological properties and pathogenicity in hamsters.This study may provide a theoretical strategy for preventing and treating the H3N8 subtype avian influenza virus-induced epidemic.METHODS:A strain of H3N8 avian influenza virus from chickens was characterised by phylogenetic analy-sis,antigenic diversity,receptor-binding specificity,neuraminidase activity,replication,and transmission in hamsters and a systematic pathological analysis was conducted.RESULTS:This novel avian influenza virus was generated through complex recombination of Eurasian avian H3 genes,North American avian N8 genes and six internal genes of H9N2 sub-type AIV.The cleavage site of the outer protein,HA,was PEKQTR↓GLF,which is characteristic of the low pathogenic avian influenza virus.The HA gene of YJ/552 exhibited the highest nucleotide homology with A/China/ZMD-22-2/2022(H3N8)at 99.09%,while the NA gene showed the highest homology with A/chicken/Dongguan/879/2022(H3N8)at 99.01%.This strain preferentially binds to avian-type receptors and could bind to human-type receptors.This virus could effectively replicate in the trachea and lungs of inoculated and contact hamsters.CONCLUSION:YJ/552 is a recombi-nant H3N8 avian influenza virus replicated in the upper respiratory system and transmitted in hamsters.This study pro-vides data support for the early warning and prevention of H3 subtype avian influenza viruses.
