Application of third-generation sequencing technique in the analysis of an-timicrobial resistance of pathogenic microorganisms
10.12138/j.issn.1671-9638.20257147
- VernacularTitle:第三代测序技术在病原微生物耐药性分析中的应用
- Author:
Peiting LIU
1
;
Jialiang ZHANG
;
Ziyang LI
;
Min HU
Author Information
1. 中南大学湘雅二医院检验医学科,长沙 410011
- Publication Type:Journal Article
- Keywords:
third-generation sequencing;
antimicrobial resistance;
Nanopore sequencing;
pathogen;
infection;
SMRT sequencing;
TGS
- From:
Chinese Journal of Infection Control
2025;24(9):1324-1332
- CountryChina
- Language:Chinese
-
Abstract:
Antimicrobial resistance poses a serious threat to human health and is the main cause for the increase in infection-related mortality.Rational and timely use of antimicrobial agents is crucial for improving the prognosis of infected patients,however,overdose and inappropriate use of antimicrobial agents can accelerate the acquisition of drug resistance of pathogenic microorganisms,promote the emergence of new resistance mechanisms,and ultimately lead to the emergence of"superbugs".Therefore,early identification of antimicrobial resistance is of significant im-portance for the rapid diagnosis and precise treatment of infectious diseases.With the development of molecular dia-gnostic techniques,third-generation sequencing(TGS)technique offers innovative solutions for detecting microbial resistance due to its advantages in long read length,easy operation,and rapid detection,significantly promoting ear-ly diagnosis and real-time monitoring of antimicrobial resistance.This review systematically summarizes the research progress of TGS in microbial resistance detection,analyzes its advantages and limitations in rapid detection of resis-tance in viruses,bacteria,and fungi,thus provides new perspectives for the early diagnosis and treatment of patho-genic microbial infections.
