Effects of Xiaoyao-Kang'ai-Jieyu formula-containing serum on BV2-HT22 cell co-culture system from mice with breast cancer-related depression
10.3969/j.issn.1000-4718.2025.09.010
- VernacularTitle:基于共培养体系探讨逍遥抗癌解郁方含药血清对体外乳腺癌相关抑郁模型BV2和HT22细胞的影响
- Author:
Song YANG
1
;
Yuanshan HAN
;
Manshu ZOU
;
Xiaoshi YU
;
Yonghong YU
;
Yuhong WANG
Author Information
1. 湖南中医药大学,湖南 长沙 410208
- Publication Type:Journal Article
- Keywords:
Xiaoyao-Kang'ai-Jieyu formula;
breast cancer-related depression;
BV2 cells;
HT22 cells;
co-culture;
glial polarization
- From:
Chinese Journal of Pathophysiology
2025;41(9):1748-1756
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of Xiaoyao-Kang'ai-Jieyu formula(XYKAJY)-containing serum on the co-culture of BV2 and HT22 cells from mice with breast cancer-related depression(BCRD).METHODS:The op-timal concentration of XYKAJY-containing serum was determined using mouse HT22 cells.In vitro co-culture model of mouse HT22 and BV2 cells was established through incubation with 4T1 cell supernatant and 200 μmol/L corticosterone.The cells were then divided into control group,model group,blank serum group,positive drug serum group,5%XY-KAJY-containing serum group,10%XYKAJY-containing serum group,15%XYKAJY-containing serum group and 20%XYKAJY-containing serum group.Alterations in HT22 cell viability was detected by CCK8 assay.The concentration level of interleukin-1β(IL-1β),IL-6 and IL-18 in the cell supernatant was quantified using enzyme-linked immunosorbent as-say(ELISA).Flow cytometry was conducted to assess the apoptosis rate of HT22 cells and quantify the expression levels of adhesion molecule CD11b on the surface of BV2 cells.Immunofluorescence was performed to determine the expression of neuron-specific enolase(NSE),synapsin 1(Syn1)and postsynaptic density protein 95(PSD95)in HT22 cells,and that of inducible nitric oxide synthase(iNOS)in BV2 cells.RESULTS:Treatment with XYKAJY-containing serum down-regulated CD11b expression level in BV2 cells(P<0.01),and reduced the release of IL-1β,IL-6 and IL-18(P<0.01).Moreover,it significantly improved the viability of HT22 cells(P<0.01),enhanced cell morphology integrity,and reduced the apoptosis rate(P<0.01).CONCLUSION:These findings indicated that XYKAJY-containing serum im-proved the function of mouse HT22 neurons by regulating the polarization phenotype of mouse BV2 cells and alleviating neuroinflammatory-induced toxicity.
