Disinfection effect of periodic hypochlorous acid on water supply pipeline and metagenomic next-generation sequencing for water specimens
10.12138/j.issn.1671-9638.20257226
- VernacularTitle:间歇性次氯酸消毒对供水管路消毒效果及宏基因组二代测序分析
- Author:
Hong ZHOU
1
;
Xiaodong GAO
;
Meixia WANG
;
Mengzhu ZHANG
;
Jun YANG
;
Jing ZHUANG
;
Hua XU
;
Wei SUN
;
Qingfeng SHI
Author Information
1. 复旦大学附属中山医院消毒供应中心,上海 200032
- Publication Type:Journal Article
- Keywords:
periodic disinfection;
biofilm;
aquatic bacteria;
metagenomic next-generation sequencing
- From:
Chinese Journal of Infection Control
2025;24(9):1244-1249
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the disinfection effect of periodic hypochlorous acid infusion on water supply pipelines.Methods Water specimens from 6 water outlets of central sterile supply department were collected at baseline for microbial culture.After introducing a slightly acidic hypochlorous acid water generator,water speci-mens were collected every 10 days.Continuous disinfection with hypochlorous acid was carried out on the 1st-3rd day,and discontinued on the 4th-10th day.Water specimens of 6 water outlets were collected on the 1st,4th,7th,and 10th day of each cycle,in total 3 cycles.Microbial culture and metagenomic next-generation sequencing(mNGS)analysis were conducted simultaneously on all water specimens from the outlets of the endoscope cleaning tank.Results Before disinfection of the pipeline,a total of 18 bottles of water specimens were collected.Bacterial count of water specimens from 10 bottles(55.56％)was between 102 and 104 CFU/mL,mainly Sphingomonas ad-hesins and Bacillus cereus.After disinfection,only 2 out of 72 water specimens(2.78％)exceeded the bacterial lim-it.The mNGS results showed that three most dominant bacterial genera in the pre-disinfection water specimens were Sphingomonas,Methylobacterium,and Ralstonia,with stringent mapped reads number(SMRN)ranging from 10 000 to 100 000;while the most dominant bacterial genera in post-disinfection water specimens were Lacto-baci-llus,Ralstonia,Acinetobacter,and Bradyrhizobium.PCoA analysis showed that there was a statistically sig-nificant difference in β-diversity of water specimens before and after disinfection(P＜0.05).Conclusion Periodic hypochlorous acid infusion disinfection can effectively remove bacterial biofilms in water pipelines,but there is a risk of bacterial biofilm recovery during disinfection suspension.In the future,monitoring and management of medical water should be strengthened.
