Mechanisms of Mycobacterium tuberculosis-induced primary cilia degra-dation in monocytes-macrophages and promotion of osteoclast differentia-tion
10.12138/j.issn.1671-9638.20252171
- VernacularTitle:结核分枝杆菌诱导单核-巨噬细胞初级纤毛降解促进破骨细胞分化的机制研究
- Author:
Chengran ZHANG
1
;
Qianfei LIU
1
;
Nanjun XIONG
1
;
Junbao CHEN
1
;
Qile GAO
1
;
Mingxing TANG
1
;
Shaohua LIU
1
Author Information
1. 中南大学湘雅医院骨科脊柱外科,湖南长沙 410008;国家老年疾病临床医学研究中心(湘雅医院),湖南长沙 410008
- Publication Type:Journal Article
- Keywords:
spinal tuberculosis;
Mycobacterium tuberculosis;
primary cilia;
osteoclast
- From:
Chinese Journal of Infection Control
2025;24(9):1208-1214
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Mycobacterium tuberculosis infection on the primary cilia of mono-cytse-macrophages and its potential mechanisms of promoting osteoclast differentiation.Methods Bone marrow-de-rived mononuclear cells(BMMCs)isolated from patients in control group and spinal tuberculosis group(TB group)were performed in vitro culture,and then cultured with Mycobacterium tuberculosis,infection model(Rv group)was constructed.Changes in cilia were observed by fluorescence staining and scanning electron microscopy tech-nique,a mouse spinal TB model was constructed for validating.Results Compared with the control group,the ex-pression of primary cili markers in the lesion of bone tissue of patients in TB group decreased significantly;After co-culturing with Mycobacterium tuberculosis,the ratio(48.56％±7.77％vs 9.58％±5.59％)and length(4.050[3.289,4.666]μm vs 0[0,0.676]μm)of primary cilia of monocytes-macrophages in the Rv group decreased sig-nificantly;The infiltration of osteoclasts in the bone marrow cavity of spinal TB mice was obvious,and the propor-tion and length of primary cilia decreased significantly.Conclusion Intracellular infection of Mycobacterium tuberculosis can induce degradation of primary cilia in monocytes-macrophages,promote osteoclast differentiation,and exacerbate vertebral bone resorption.
