Sodium tanshinone ⅡA sulfonate inhalation attenuates pulmonary hyper-tension associated with chronic obstructive pulmonary disease in mice
10.3969/j.issn.1000-4718.2025.09.006
- VernacularTitle:雾化吸入丹参酮ⅡA磺酸钠对小鼠慢性阻塞性肺疾病相关肺动脉高压的治疗作用
- Author:
Runhua HOU
1
;
Siting WANG
;
Hongping ZHANG
;
Ruanyuan YE
;
Zeping YANG
;
Mengzhu LI
;
Guanqing ZHEN
;
Jiarui FAN
;
Chunyun ZHANG
;
Rui CHEN
;
Defu LI
Author Information
1. 广州医科大学附属第五医院重症医学科/广东高校生物靶向诊治与康复重点实验室,广东 广州 510700
- Publication Type:Journal Article
- Keywords:
sodium tanshinone ⅡA sulfonate;
chronic obstructive pulmonary disease;
pulmonary hyperten-sion;
interleukin-6;
inflammation
- From:
Chinese Journal of Pathophysiology
2025;41(9):1713-1720
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of nebulized sodium tanshinone ⅡA sulfonate(STS)in a mouse model of pulmonary hypertension associated with chronic obstructive pulmonary disease(COPD-PH).METHODS:A to-tal of 32 healthy SPF-grade male C57BL/6 mice were randomly divided into 4 groups:control(CTL,n=8)group,COPD-PH(CS+LPS,n=8)group,STS-treated COPD-PH(CS+LPS+STS,n=8)group,and STS(n=8)group.The COPD-PH model was established through whole-body exposure to cigarette smoke(CS)combined with lipopolysaccharide(LPS)in-halation.Mice were subjected to cigarette smoke exposure in a chamber(9 cigarettes/h,2 h/session,2 sessions/d,6 d/week)for 60 d,except on days of LPS inhalation.On days 1 and 14,COPD-PH model mice received LPS(7.5 μg/mouse in 50 μL saline)via intranasal inhalation,while the CTL and STS groups received an equivalent volume of saline.STS was administered via nebulized inhalation(5 mg/kg,30 min per session,twice daily)immediately before CS exposure.At the end of the modeling period,lung function and right heart pressure were assessed.Bronchoalveolar lavage fluid(BALF)was collected for inflammatory cell counting.Levels of interleukin-6(IL-6)in BALF supernatants and plasma were measured using ELISA.Pathological changes in the airway and lung tissues were evaluated.RESULTS:(1)Com-pared to CTL mice,those exposed to CS and LPS exhibited lesions characteristic of COPD-PH,including emphysema,lung inflammation,decreased lung function,and increased right ventricular systolic pressure(RVSP)and right ventricu-lar hypertrophy index(RVHI)(P<0.05);(2)COPD-PH mice showed significantly elevated IL-6 levels in both BALF and plasma(P<0.05);(3)STS treatment alleviated emphysema and lung inflammation,improved lung function,prevent-ed increases in RVSP and the RV/(LV+S)ratio,and reduced IL-6 levels in both BALF and plasma(P<0.05).CON-CLUSION:The results indicate that nebulized inhalation of STS significantly slows the progression of COPD-PH,likely due to its ability to inhibit lung inflammation and reduce IL-6 expression in the lungs.
