Chromosomal polymorphisms: the new indication for PGT-A?
10.3760/cma.j.cn101441-20220916-00400
- VernacularTitle:染色体多态性：PGT-A新指征？
- Author:
Xiao BAO
1
;
Hao SHI
1
;
Yingpu SUN
1
Author Information
1. 郑州大学第一附属医院生殖医学中心，河南省生殖与遗传重点实验室，郑州 450052
- Publication Type:Journal Article
- Keywords:
Pregnancy outcome;
Chromosomal polymorphisms;
Preimplantation genetic testing for aneuploidies;
Embryo aneuploid;
Copy number variation of abortion tissue
- From:
Chinese Journal of Reproduction and Contraception
2022;42(11):1107-1113
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore whether the chromosomal polymorphism variation is the new indication of preimplantation genetic testing for aneuploidies (PGT-A).Methods:Clinical data of the patients who received PGT-A frozen-thawed embryo transfer and in vitro fertilization-embryo transfer (IVF-ET) fresh embryo transfer from the Center for Reproductive Medicine of the First Affiliated Hospital of Zhengzhou University from January 1, 2012 to December 31, 2021 were analyzed in a retrospective cohort study. According to the karyotype of the couples, PGT-A cycles were divided into female, male and couple chromosome polymorphism variation groups, and couples with normal chromosomes at the same time were included in control group. IVF cycles were divided into female and male chromosome polymorphism variation groups, and the couples with normal chromosomes at the same time were included in control group. The laboratory results and pregnancy outcomes were compared among the groups. Results:In PGT-A cycles, there were no significant differences in chromosome aneuploidy rate, clinical pregnancy rate after frozen-thawed embryo transfer, early abortion rate and live birth rate among the four groups (all P>0.05). In IVF fresh embryo transfer cycles, there were no significant differences in clinical pregnancy rate, early abortion rate and live birth rate among the three groups (all P>0.05). Among the early abortion patients after IVF cycles, the abnormal rates of copy number variation (CNV) in the abortion tissues of the female chromosome polymorphism variation group, the male chromosome polymorphism variation group and the normal control group were 26.67% (4/15), 57.89% (11/19) and 64.59% (363/562), respectively, with a statistical difference ( P=0.010). Conclusion:Chromosomal polymorphism of couples does not affect the aneuploidy rate of embryos, and does not affect the pregnancy outcomes of PGT-A frozen-thawed embryo transfer cycle and IVF fresh embryo transfer cycle, and does not increase the chromosome abnormality rate of abortion tissues. At present, there is no clear evidence to support that chromosomal polymorphisms as a new indication of PGT-A.
