Metabolomics Analysis in the Classification and Gender Differences of Acne Vulgaris
10.13241/j.cnki.pmb.2025.15.001
- VernacularTitle:痤疮分级和性别差异的代谢组学研究
- Author:
Lu GAO
1
;
Yuan-yuan ZHU
;
Min GAO
;
Tian-shu YANG
Author Information
1. 复旦大学代谢与整合生物学研究院 上海 200438
- Publication Type:Journal Article
- Keywords:
Acne vulgaris;
Metabolomics;
Biomarkers
- From:
Progress in Modern Biomedicine
2025;25(15):2401-2410
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study applies metabolomics technology integrated with bioinformatics analysis to investigate the distinctive characteristics and differential expressions of serum lipids in acne patients across disease stages and between genders.The research aims to identify potential biomarkers,thereby providing innovative perspectives and theoretical foundations for the complex pathogenesis of acne.Methods:From November 2021 to August 2022,serum samples were collected from 41 acne patients with mild,moderate,and severe acne,as well as 26 healthy controls,at the Department of Dermatology,First Affiliated Hospital of Anhui Medical University.Serum lipidomics analysis was performed using liquid chromatography-mass spectrometry(LC-MS).Multivariate statistical analysis of differentially expressed metabolites was conducted using partial least squares discriminant analysis(PLS-DA).Metabolomics data were further analyzed using volcano plots and Gene Ontology(GO)pathway enrichment analysis to identify significantly altered signaling pathways across disease stages and genders,as well as key candidate biomarkers.Results:This study revealed distinct serum metabolite profiles among acne patients at different disease stages.Throughout the progression of acne,the purine pathway remains consistently upregulated.However,distinct dysregulated pathways characterize different severity stages:the purine pathway exhibits the most pronounced alterations in mild and moderate groups,while the methionine metabolism pathway demonstrates the most significant upregulation in severe cases.Males exhibited prominent dysregulation of the purine pathway,whereas females showed significant alterations in arginine and proline metabolism.Additionally,we identified nine metabolites common across all stages,six stage-specific metabolites,and nine gender-specific metabolites in male and female patients,respectively.Conclusions:Acne patients exhibit distinct metabolic profiles across disease stages and genders.The identification of both common metabolic biomarkers shared across stages and or gender specific biomarkers offers novel pathways for precise phenotyping and personalized therapeutic approaches.
