Liuwei-Dihuang decoction modulates ovarian function in POF mice via the Notch signaling pathway
10.3969/j.issn.1000-4718.2025.10.016
- VernacularTitle:六味地黄汤通过Notch信号通路调控卵巢早衰小鼠卵巢功能
- Author:
Xia LIANG
1
;
Nina LI
;
Haifeng YE
;
Xiaoting YANG
;
Liaoliao HU
Author Information
1. 衢州职业技术学院,浙江 衢州 324000
- Publication Type:Journal Article
- Keywords:
premature ovarian failure;
follicular development;
Liuwei-Dihuang decoction;
Notch signaling pathway
- From:
Chinese Journal of Pathophysiology
2025;41(10):2009-2015
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the therapeutic effects of Liuwei-Dihuang decoction(LD)on ovarian function in pre-mature ovarian failure(POF)mice,and to uncover the role of the Notch signaling pathway in its mechanism.METHODS:A mouse model of POF was constructed through intraperitoneal injection of cyclophosphamide/busulfan.Subsequently,the mice were randomly divided into six groups(n=12 per group):control,POF model,low-dose LD(LD-L),medium-dose LD(LD-M),high-dose LD(LD-H),and positive drug(0.7 mg/kg diethylstilbestrol)groups.The ovarian histopathologi-cal alterations were assessed by hematoxylin-eosin(HE)staining.Serum levels of follicle-stimulating hormone(FSH),anti-Müllerian hormone(AMH),and estradiol(E2)were quantified via ELISA assays.Immunohistochemistry(IHC)was conducted to compare the expression level of cell proliferation marker Ki-67 in mouse ovarian tissues among the groups.The expression of proteins involved in the Notch pathway components[Notch1,Notch2,Jagged1(JAG-1),and hairy and enhancer of split 1(Hes1)]was determined via IHC and Western blot,while the corresponding mRNA levels of Notch1,Notch2,JAG-1,JAG-2,Hes1,and Hes5 were quantified through the qPCR analysis.RESULTS:Ovarian weight was significantly higher,and the number of atretic follicles was significantly lower in the LD-H and positive drug groups com-pared to the model group(P<0.05).However,serum FSH levels were decreased(P<0.01),whereas those of E2 and AMH were elevated in both treatment groups compared with the model group(P<0.01).In the LD-H group,the expres-sion of key molecules of the Notch pathway components was increased at the protein and mRNA level compared with the model group(P<0.01).CONCLUSION:LD ameliorates ovarian dysfunction in POF mice via activating the Notch sig-naling pathway.
