miR-200a inhibits formation of primary cilia and decreases X-ray-induced apoptosis resistance in rat cardiac fibroblasts
10.3969/j.issn.1000-4718.2025.10.006
- VernacularTitle:miR-200a抑制初级纤毛的发生并促进X线辐射诱导的大鼠心脏成纤维细胞凋亡
- Author:
Li MA
1
;
Xiaoni MA
;
Songbo FU
;
Chengxu MA
Author Information
1. 兰州大学第一临床医学院,甘肃 兰州 730000
- Publication Type:Journal Article
- Keywords:
microRNA-200a-3p;
cardiac fibroblasts;
X-ray;
apoptosis;
primary cilia
- From:
Chinese Journal of Pathophysiology
2025;41(10):1920-1925
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of microRNA-200a(miR-200a)on the formation of primary cilia and the apoptosis in X-ray-injured cardiac fibroblasts.METHODS:Hearts were isolated from neonatal Wistar rats and di-gested with trypsin.Cardiac fibroblasts were isolated,irradiated with 1 Gy of X-ray,and subsequently transfected with miR-200a-3p mimic or si-IFT88.Primary cilia were detected by immunofluorescence staining,and tranfoming growth fac-tor-β1(TGF-β1)in the culture medium was detected by ELISA.The viability of cardiac fibroblasts was detected by CCK-8.The mRNA expression of tumor necrosis factor-α(TNF-α)and caspase-3 was detected by RT-qPCR,and the protein expression of TNF-α,caspase-3,collagen type I α1 chain(Col1α1)and TGF-β1 was detected by Western blot.RE-SULTS:Compared with control group,X-ray irradiation significantly decreased the expression of miR-200a-3p and the level of apoptosis,but increased the proportion of cells with primary cilia,the length of primary cilia and the viability of cardiac fibroblasts.Compared with X-ray group,the percentage of apoptotic cardiac fibroblasts in si-IFT88 group was in-creased.Compared with X-ray group,the proportion of cells with primary cilia and the TGF-β1 content in cardiac fibro-blasts were reduced,and the proportion of apoptotic cells increased in miR-200a-3p overexpression group.CONCLU-SION:X-ray-induced down-regulation of miR-200a-3p in neonatal rat cardiac fibroblasts drives the formation of primary cilia and suppresses the apoptosis.
