Effects of CD147 deletion in segments 4-5 of epididymis on sperm maturation
10.3760/cma.j.cn101441-20220126-00044
- VernacularTitle:附睾4-5段CD147缺失对精子成熟的作用研究
- Author:
Jiawei TIAN
1
;
Pengyuan DAI
1
;
Hao CHEN
1
Author Information
1. 南通大学医学院 生殖医学研究院，南通 226001
- Publication Type:Journal Article
- Keywords:
Sperm maturation;
Fertilization in vitro;
CD147;
Segment 4-5 of epididymis
- From:
Chinese Journal of Reproduction and Contraception
2022;42(10):995-1002
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the potential role of CD147 expressed in the mouse epididymal epithelial cells on sperm maturation.Methods:Lcn5-Cre mice which were specifically expressed in the principal cells in the segments 4-5 of mouse epididymis were bred with CD147 flox/flox to obtain 24 of the CD147 conditional knockout mice (CD147 cKO-Lcn5) and its counterpart CD147 flox/flox control mice. Computer-aided sperm analysis (CASA), A23187-induced sperm acrosome reaction, and in vitro fertilization (IVF) were used to evaluate the effects of CD147 on sperm functions. Results:After CD147 knockout in the mice segment 4-5 of the caput epididymis, there were no statistically significant differences in sperm motility and acrosome reaction. Of note, the two-cell ratio of CD147 cKO-Lcn5 mice (74.03%±2.39%) was significantly reduced compared with that of CD147 flox/flox mice (90.59%±2.39%) in the experiment for IVF ( P=0.012). Conclusion:CD147 deleted in the 4-5 caput epididymis has limited regulatory effects on sperm motility and acrosome reaction processes, but significantly affected the ratio of two-cell after IVF.
