Allicin alleviates senna-induced diarrhea in mice through modulation of inflammation and oxidative stress
- VernacularTitle:大蒜素通过改善炎症和氧化应激缓解番泻叶诱导小鼠腹泻的机制
- Author:
Qing ZHOU
1
;
Jia-min WU
;
Mo GUO
;
Yao-yu ZHAO
;
Lei HUANG
;
Fei GE
;
Pang-bo YANG
;
Yuan-yuan QIN
;
Yu WANG
;
Jun GUO
;
Shan GAO
Author Information
- Publication Type:Journal Article
- Keywords: allicin; diarrhea; senna leaf; anti-inflam-mation; anti-oxidative stress; aquaporin; tight junction
- From: Chinese Pharmacological Bulletin 2025;41(10):1906-1914
- CountryChina
- Language:Chinese
- Abstract: Aim To study the therapeutic effect of al-licin on senna-induced diarrhea in mice and to explore the underlying mechanism.Methods Forty-eight C57BL/6J mice were randomly divided into six groups:control,model,loperamide positive control group(2 mg·kg-1),allicin low-dose group(6 mg·kg-1),allicin medium-dose group(12 mg·kg-1)and allicin high-dose group(18 mg·kg-1).Except for the con-trol group,the diarrhea model was induced in the other groups by intragastric administration of senna leaf ex-tract.After drug administration,several diarrhea indi-ces were measured:the rate of loose stools,diarrhea index,accumulated frequency of loose stools at differ-ent time points within 5 hours,and small intestine pro-pelling rate.Serum levels of TNF-α and IL-6 were de-tected by ELISA.Serum NO content was determined u-sing the Griess method.The activities of SOD and CAT,as well as MDA content in the ileum and colon,were measured.The pathological changes and the ex-pression of mRNA related to intestinal barrier proteins in the ileum and colon were evaluated using HE stai-ning and RT-qPCR.Results Allicin improved diar-rhea symptoms in mice induced by senna leaf.It re-duced the rate of loose stools,diarrhea index,cumula-tive number of loose stools in five hours,and the intes-tinal propulsion rate.Allicin also protected the intesti-nal mucosa,decreased serum TNF-α and IL-6 levels,and lowered MDA content in the intestines.It in-creased serum NO levels and enhanced SOD and CAT activities in the intestines.Additionally,allicin upreg-ulated the mRNA expression of AQP1,AQP4,and ZO-1 in intestinal tissues.Conclusions Allicin has a significant therapeutic effect on senna-induced diarrhea in mice.The underlying molecular mechanisms may involve anti-inflammatory and antioxidant effects,in-creased NO content,and upregulation of mRNA ex-pression of aquaporins and tight-junction proteins.
