Luteolin alleviates liver fibrosis by inhibiting autophagy of hepatic stellate cells
- VernacularTitle:木犀草素抑制肝星状细胞自噬缓解肝纤维化的作用及机制
- Author:
Shu-ling CHEN
1
;
Xi-xuan WANG
;
Rui-qi LI
;
Da-wei YANG
;
Hui CAO
;
Yong-feng YANG
Author Information
- Publication Type:Journal Article
- Keywords: luteolin; liver fibrosis; hepatic stellate cells; p62; LC3; rapamycin
- From: Chinese Pharmacological Bulletin 2025;41(10):1875-1883
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the mechanism of luteolin in alleviating hepatic fibrosis.Methods C57BL/6 mice were randomly divided into the control group,CCl4 group,silybin group(100 mg·kg-1)and luteo-lin group(100 mg·kg-1).After 10-week modeling and 2-week treatment,the serum levels of aminotrans-ferase and liver histopathology were examined.Hepatic fibrosis and autophagy-related gene expression were as-sessed using immunohistochemistry and immunofluores-cence.Human hepatic stellate cell line(LX2)was cultured and divided into control,TGF-β1(10 mg·L-1),TGF-β1+silybin(40 μmol·L-1),TGF-β1+luteolin(40 μmol·L-1).Fibrotic and autophagy-re-lated markers were analyzed using quantitative real-time PCR,Western blot,immunofluorescence and MDC staining.Results Compared with the CCl4 group,the treatment groups showed significantly improved liver function and reduced hepatic fibrosis,with markedly downregulated COL1A1 and α-SMA expression,and luteolin demonstrated superior efficacy.Compared with TGF-β1 group,luteolin treatment significantly de-creased mRNA levels of COL1A1,ACTA2 and MAP1LC3B,while increasing the mRNA level of SQSTM1,the protein levels of COL1A1 and α-SMA de-creased,p62 was enhanced,the LC3Ⅱ/Ⅰ ratio was downregulated,and autophagy was reduced.These effects of luteolin were reversed by autophagy inducer rapamycin.Conclusion Luteolin alleviates liver fi-brosis by decreasing the autophagy of hepatic stellate cells.
